Biotechnological methods can be used in dairy cow reproduction to increase reproductive potential and improve economic outcomes of milk production. Biotechnological methods widely used in reproduction include artificial insemination and embryo transfer, and more recently insemination with sexed semen, which makes it possible to control the sex of the offspring. This study presents an analysis of the effectiveness of the use of sexed semen in a selected herd of dairy cows. The results confirmed the effectiveness of sexed semen at increasing births of female calves. Sexed semen was most effective among heifers. In that group the fewest insemination doses were required for conception (1.54), and pregnancy after the first insemination procedure was noted in 52.2% of cases. The values of selected fertility parameters (calving-to-first-service interval, service period, services per conception, and rate of conception after first service) did not deviate significantly from recommended values in cows inseminated with sexed and unsexed semen. Among the group of cows inseminated with sexed semen, fertility parameters were significantly more favourable than in the group inseminated with conventional semen.

This research sought to determine the effect of selected milk protein polymorphisms on the reproduction parameters of 598 black and white Polish Holstein–Friesian cattle. The analyzed genes were kappa-casein (CSN3) and beta-lactoglobulin (BLG). The following reproductive indexes were selected: the age at first calving, the interpregnancy period, the calving interval, and the insemination index. The influence of the identified genotypes on the course of parturition in cows was also analyzed. Source data were collected for each cow from the beginning of the herd life and reproduction to its culling from the herd or the end of its third lactation. Data on the age at first calving, the amount of semen portions for artificial insemination (insemination index), the interpregnancy period, and the calving interval for each cow were also collected. A contingency analysis was performed through contingency tables using a Pearson’s chi-squared test for each CSN3 and BLG genotype. The results show that the BB genotype of the kappa-casein gene was associated with the most favorable values of reproductive indicators. In the case of the calving interval, the values were significantly more favorable than those of other genotypes (p ≤ 0.05). No effect of beta-lactoglobulin polymorphism on the analyzed reproductive indices was observed. On the other hand, in the case of the interpregnancy period, significant statistical differences were obtained between the AA and BB genotypes. The analyzed kappa-casein and beta-lactoglobulin genotypes did not significantly influence the course of parturition in cows. To conclude, the genotype polymorphism BB CSN3 is the most favorable for the performance of the cows in the examined herd.