SUMMARYAn intramuscular dose of 12 mg/kg of this steroid anaesthetic produced sedation in cynomolgus monkeys, and higher doses (up to 60 mg/kg) produced light anaesthesia lasting up to 3 hours. Surgical anaesthesia could be induced by a single injection of 120 mg/kg, or by an intramuscular injection of 18 mg/kg followed by 6-12 mg/kg intravenously.No clinical or pathological effects were observed after daily use of the anaesthetic for 4 weeks.

Abstract. Entire and castrated male pigs were actively immunised against the steroid 5α-androst-16-en-3-one (androstenone) conjugated to bovine thyroglobulin as its carrier protein. This immunogen was incorporated with either Freund's complete adjuvant or a water-in-oil emulsion adjuvant; only the latter was found to be acceptable in the pig tissue. Levels of free and antibody-bound androstenone were measured in the plasma of immunised and control boars. In the controls, levels of free androstenone rose from 15 ng/ml at 13 weeks old to 36 ng/ml at 27 weeks. In the immunised boars, levels of free steroid fell from 16 ng/ml at 13 weeks old to zero at 17 weeks old while levels of antibody-bound androstenone rose from zero at 13 weeks old to a mean level of 70 ng/ml at 27 weeks. When immunised and control boars and non-immunised gilts were killed at 135 kg live weight, the levels of androstenone in their carcass fat were measured. Their meat was also subjected to organoleptic testing at the Meat Research Institute, Langford. Meat from animals whose fat contained significant levels of androstenone (0.4–1.6 μg/g) was found to taste of 'boar taint' while meat from control gilts with low fat levels of the steroid (0.1–0.2 μg/g) tasted normal. The uptake of [3H]androstenone was compared in 12 separate body tissues of one immunised and one control boar. No significant differences were found. The presence of specific antibody in the immunised boar had not inhibited movement of steroid between plasma and body tissue. Some hypotheses raised by these findings are discussed and the likelihood of successfully using immunisation against 'boar taint' is examined and questioned.

No abstract

To assess the effect of abnormal control of bursa-derived B cell function upon the development of spontaneous autoimmune disease, newly hatched chicks were surgically bursectomized or sham-bursectomized and studied up to 4 months of age. The presence of autoimmune disease was assessed by direct Coombs' test, measurement of antinuclear antibody, total hemoglobin, packed cell volume, plasma bilirubin, red cell survival, and immunofluorescence studies on frozen kidney sections. Despite abnormal immunoglobulin levels detected in bursectomized chickens, no significant differences in terms of autoimmune activity could be demonstrated between the two groups. This suggests that primary B cell control is an unlikely factor in the etiology of autoimmune disease and supports earlier studies which imply that a major factor leading to progressive autoimmune disease is the failure of a normal T cell suppression function.