Soybean bacterial pustule is caused by the gram‐negative bacterium Xanthonomas citri pv. glycines. The identification of sources of resistance for use in molecular breeding is a challenge due to the existence of complex interactions between pathogenic strains and soybean cultivars. This study aimed to evaluate the reaction of 118 soybean cultivars to inoculation with strains 2440P and 2447 of X. citri pv. glycines and to identify molecular markers associated with the resistance. The genome‐wide association study approach was used to identify association between the phenotype and the single nucleotide polymorphisms (SNP) markers. The positions of significant SNPs were mapped to the soybean Williams 82 genome, and protein‐coding genes near them were identified. One and 116 cultivars were resistant to strains 2440P and 2447, respectively. Eleven SNPs were significantly associated with resistance to strain 2440P and five to 2447. Two significant SNPs are predicted to result in amino acid changes in benzyl alcohol O‐benzoyltransferase‐like and MAIN‐LIKE 1‐like proteins, which after confirmation, could be used in marker‐assisted selection in order to increase the frequency of resistant alleles in new cultivars.
Maize (Zea mays ssp. Mays) is a widely cultivated crop, having one of the highest productivities among cereals, and it is of great importance in human consumption, both in natura and processed. In addition, it has applications in industry as a source of energy through corn ethanol and animal feed. Many diseases can affect maize yield such as the Maize Common Rust (MCR) (Puccinia sorghi Schwein), a leaf disease which causes the appearance of pustules. The aim of this study was to classify maize lines between resistant and susceptible, selecting 50% of them to be carried on the breeding pipeline. A dataset containing three time-point evaluations in two years using a visual score scale and two Unmanned Aerial Vehicle (UAV) - couple sensors (multispectral and thermal) data were analyzed with six machine learning algorithms in order to identify the training time set to deliver the best classification performance. The three time-point evaluations phenotypic data along with the genetic markers data were used to explore the performance of the Support Vector Machine (SVM) and the Artificial Neural Network (ANN) algorithms in a k-fold cross-validation analysis with nine datasets. Their learning curves and feature importance rank were analyzed using the SVM algorithm. Our results showed that the last evaluation training set delivered the highest accuracies, of approximately 80 per cent, with Logistic Regression and SVM outperforming the other algorithms. The results obtained with the analysis by year suggest that a homogenous distribution of scores is of great importance for an effective MCR resistance classification. Our results also demonstrated the advantageous use of the SVM algorithm, in which models had the capacity to generalize using a smaller number of features. Similar performance metrics were achieved with SVM when the third evaluation and the three time-point evaluations combined together were employed. The SVM learning curves indicate that the addition of more training samples would be beneficial for all datasets analyzed. The five most important features for each dataset were listed, resulting in a predominance of the Red wavelength in the first position of the rank. In addition, the protein- coding genes aligned with the markers’ allele sequence ranked as important should be further explored in genomic-functional studies. Keywords: Maize common rust. Machine learning. SVM. ANN. Data mining.
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