Introduction: The present study aimed to assess the seroprevalence and spatial distribution of Neospora caninum and Toxoplasma gondii in dogs. Methods: Blood samples (n = 241) were collected and analyzed for the presence of anti-N. caninum and anti-T. gondii antibodies. The spatial distribution was evaluated using kernel density estimation (KDE). Results: Anti-N. caninum and anti-T. gondii antibodies were detected in 24.06% (58/241) and 9.54% (23/241) of samples, respectively. A heterogeneous spatial distribution of positive dogs was observed across the city. Conclusions: These data are pivotal for better understanding the dynamics of infection caused by these protozoa in the canine population.
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