The purpose of this study was to investigate whether LED light at different wavelengths affects the expression profile of 143 cancer predisposition genes in both diabetic and normal human fibroblasts. In this study, both diabetic and normal fibroblast cell lines were cultured and irradiated with red (635 nm), green (520 nm), and blue (465 nm) LED light for 10 minutes at 0.67 J/cm2 each. After that, mRNA from all cell lines was extracted for microarray analysis. We found that green light activates EPHB2, KIT, ANTXR2, ESCO2, MSR1, EXT1, TSC1, KIT, NF1, BUB1B, FANCD2, EPCAM, FANCD2, NF, DIS3L2, and RET in normal fibroblast cells, while blue and red light can upregulate RUNX1, PDGFRA, EHBP1, GPC3, AXIN2, KDR, GLMN, MSMB, EPHB2, MSR1, KIT, FANCD2, BMPR1A, BUB1B, PDE11A, and RET. Therefore, genetic screening before phototherapy treatment may be required.

The effects of LED light at 635 nm in fibroblast and osteoblast were investigate in fundamental changes as preliminary experiment. In this study, primary gum fibroblast cells and osteoblast cell line were cultured. After LED light irradiation, we found that fibroblast growth rate and cell proliferation are increased after irradiated by 630nm LED light. Optimum energy for wound healing is 0.34 J/cm 2 at 630 nm. Optimum energy for proliferation is 0.67 J/cm 2 at 630 nm. However, osteoblasts do not significantly and effectively response to LED light at 630 nm.

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