Fibrinolytic enzymes are agents that dissolve fibrin clots. These fibrinolytic agents have potential use to treat cardiovascular diseases, such as heart attack and stroke. The aim of the study was to purify fibrinolytic enzyme from the marine isolate, Pseudoalteromonas sp., IND11. Enzyme was purified to electrophoretic homogeneity using ammonium sulphate precipitation, ion exchange and affinity chromatography. The SDS-PAGE showed that it was a monomeric protein with an apparent molecular weight of 64 kDa. The purified enzyme was active at pH 6.0-9.0 with an optimum pH of 8.0. It was stable upto 50°C, exhibiting maximum activity between 30 and 60°C. Among the ions, Na + and Ca 2+ activated enzyme activity. The Fe 2+ did not obviously activate or inhibit the enzyme activity. The ions such as Cu 2+ , Hg 2+ and Zn 2+ strongly affected enzyme activity. This enzyme activated plasminogen and also had direct clot lytic activity. It digested the fibrin net of blood clot, suggests its potential as an effective thrombolytic agent. This study explores new sources of fibrinolytic enzymes to treat and prevent CVDs.

A low-cost culture medium for the production of bacterial alkaline protease was developed after response surface methodological approach. Central composite design was applied to optimize the concentration of the three significant factors, namely pH, sucrose and yeast extract. A second-order model equation was obtained and then validated experimentally. The model adequacy was highly satisfactory as the coefficient of determination was 0.98. The maximum enzyme production was 2213 U mLG 1 after 72 h of incubation, which showed about fivefold increase in enzyme production than unoptimized medium. The partially purified enzyme showed optimum activity at 50°C at pH 9.0. Crude enzyme exhibited a significant stability with most of the tested commercial laundry detergents. This enzyme removed the gelatin layer of used X-ray film effectively. Considering its promising properties, Bacillus cereus IND6 enzymatic preparations may be considered a potential candidate for detergent applications and silver recovery.

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