BACKGROUND The aim of the study is to evaluate the effectiveness of subcutaneous pre-incisional and mesosalpinx infiltration of 0.5% bupivacaine in post-operative pain relief. MATERIALS AND METHODS This is a randomised placebo-controlled double-blind study conducted at Bangalore Medical College and Research Institute on 50 patients scheduled for elective tubal ligation by minilaparotomy under general anaesthesia from April to October 2014. Patients were randomised into 2 groups of 25 each by computer generated random list. Group A patients received 0.5% bupivacaine 10 cc pre-incisional subcutaneous and 5 cc in the mesosalpinx, while Group B patients received normal saline infiltration. In this study, the intensity of post-operative pain was assessed using Verbal Rating Scale (VRS). Data collection was done using questionnaire and collected data was analysed by Student's t-test, Chi-square and Fisher Exact test using SPSS software and descriptive statistical programme, p value < 0.01 was considered significant. RESULTS 50 patients were studied in two groups. Age and weight of the patients were comparable between 2 groups. Pain score in recovery room 2, 4, 6, 8, 10 hours post-surgery were significantly less in bupivacaine group (p < 0.005). Time for rescue analgesia is 3.92 ± 1.07 hrs. in Group A compared to 1.32 ± 0.54 in Group B with p < 0.01. CONCLUSION Pre-incisional and mesosalpinx infiltration of 0.5% bupivacaine in abdominal tubectomies by minilaparotomy led to significant reduction of post-operative pain with prolonged period for rescue analgesia.