Sinking Creek (HUC 06010103046), in the Watauga watershed of northeast Tennessee, is impaired due to Escherichia coli. To assess how E. coli and other water quality parameters fluctuated during storm events, water samples were collected with automated samplers during eight storms at two locations: Sinking Creek and a feeder spring. Turbidity and electrical conductivity data loggers were deployed in the creek, and dissolved oxygen (DO) was measured in situ. The presence of optical brighteners, used in detergents and an indicator of residential wastewater, was assessed using cotton fabric deployed at both sites and analyzed by an external laboratory. The Colilert Quanti-Tray method was used to process water samples for E. coli. Relationships between water quality parameters and lagged precipitation were assessed using crosscorrelation. At the creek, E. coli and turbidity increased within 2 h of precipitation, exceeding the single sample water quality standard of 941 cfu 100 ml −1 during the storm. At the spring, E. coli became elevated more quickly than at the stream, within 30 min of precipitation, and decreased below the standard during the event. Electrical conductivity decreased within 1.5 h of the storm at the creek, and DO levels were higher at the creek than at the spring. Optical brightener analysis indicated possible presence of residential wastewater during one of two sampled storms. Targeted sampling and dye tracing are recommended to validate this hypothesis. These results may be used to inform field methods in similar storm sampling studies and will be useful in watershed restoration efforts in Sinking Creek. 1 INTRODUCTION Water quality in rivers and streams in the United States has been affected by channelization, oxygen depletion, and agricultural runoff, resulting in impairment of 187,872 stream and river miles and causing loss of habitat for aquatic life (USEPA, 2016). The two most common impairments in Ten