In this study, we investigated whether the nanofibers produced by natural-synthetic polymers can probably promote the proliferation of co-cultured adipose-derived stem cells/human fibroblast cells (ADSs/HFCs) and synthesis of collagen. Nanofiber was fabricated by blending gelatin and poly (L-lactide co-ɛ-caprolactone) (PLCL) polymer nanofiber (Gel/PLCL). Cell morphology and the interaction between cells and Gel/PLCL nanofiber were evaluated by FESEM and fluorescent microscopy. MTS assay and quantitative real-time polymerase chain reaction were applied to assess the proliferation of co-cultured ADSs/ HFCs and the collagen type I and III synthesis, respectively. The concentrations of two cytokines including fibroblast growth factor-basic and transforming growth factor-β1 were also measured in culture medium of co-cultured ADSs/HDCs using enzyme-linked immunosorbent assay assay. Actually, nanofibers exhibited proper structural properties in terms of stability in cell proliferation and toxicity analysis processes. Gel/PLCL nanofiber promoted the growth and the adhesion of HFCs. Our results showed in contact co-culture of ADSs/HFCs on the Gel/PLCL nanofiber increased cellular adhesion and proliferation synergistically compared to non-coated plate. Also, synthesis of collagen and cytokines secretion of co-cultured ADSs/HFCs on Gel/PLCL scaffolds is significantly higher than non-coated plates. To conclude, the results suggest that Gel/PLCL nanofiber can imitate physiological characteristics in vivo and enhance the efficacy of co-cultured ADSs/HFCs in wound healing process.

No abstract

Bioethanol is a promising candidate and the eco-friendly material for reducing greenhouse gases and it can be a good alternative to fossil fuels. We describe a development of framework for green fuel production. The production of bioethanol as a fuel has been investigated from sugarcane scum (bagasse, which can be hydrolyzed and fermented by high carbon content). In this process, carbohydrates were converted into monomers and then by fermentation of these monomers leads to the production of ethanol. Samples were prepared at different experimental conditions (Saccharomyces and acidic media for hydrolyzing) to carry out the fermentation process. Finally, identification and characterization of ethanol for the best condition was performed by GC-MS (500 gr bagasses, 0.25 gr yeast in acidic media and boiling temperature). The use of cheap yeast is a novelty of this work because it provides commercialization of bioethanol production which is very important in ethanol generation.