Sheep were fed mulga (Acacia aneura) ad libitum and supplemented with 0, 12 or 24 g/day of polyethylene glycol molecular weight 4000 (PEG), with or without an additional supplement of 1.5 g/day of each of nitrogen (N), phosphorus (P) and sulfur (S) (NPS). Supplementation with either 12 g/day PEG or 24 g/day PEG increased dry matter intake by 56 and 78% respectively and clean wool growth per unit surface area by 166 and 178% respectively. Supplementation with 24 g/day PEG increased wool fibre diameter, linear growth rate and volumetric growth rate by 32, 29 and 143% respectively. Sheep receiving 24 g/day PEG retained more N (1.80 g/day) and S (254 mg/day) and digested more N (218 g/kg) and S (229 g/kg) when compared with non-supplemented animals. These parameters were further enhanced when NPS was supplemented in conjunction with 24 g/day PEG. Maximum precipitation (77%) of mulga condensed tannins (CT) occurred at a PEG to CT ratio of 0.7:l.
Sixteen mature, ruminally cannulated wethers (average BW = 41 +/- 1 kg) were fed a low-quality hay diet with or without a cottonseed meal (CSM) supplement and the parasympathomimetic agonist slaframine (SF). Treatments were basal diet (Mitchell grass hay, 4.8% CP, 46.8% ADF) available on an ad libitum basis, basal diet plus SF (8 micrograms/kg BW, 2 x daily i.m. injection), basal diet plus CSM (41.0% CP; 100 g/d), or basal diet plus SF and CSM. Treatments were arranged as a 2 x 2 factorial within a replicated 4 x 4 Latin square with 20-d periods followed by a 10-d adjustment during which only the basal diet was fed. All measurements were performed within the final 10 d of each period. Slaframine increased salivary flow by 10 to 35% (P < .07), ruminal fluid dilution rate by 8 to 11% (P < .10), and pH by 3 to 4% (P < .001). A twofold increase (P < .05) in ruminal cellulolytic bacteria numbers occurred in SF-treated wethers. Despite these SF-induced changes in the ruminal environment, whole-tract apparent nutrient digestibility, N and mineral balance, and ruminal VFA concentrations were not changed. Cottonseed meal increased forage intake by 34 to 54% (P < .001) and DM digestibility by 30% (P < .001). Cottonseed meal supplementation of a Mitchell grass hay diet improved nutritional status and attenuated live weight loss.
A 3-year experiment was conducted at Kairi Research Station on the Atherton Tablelands, Queensland, to determine the effects of stocking rate and applied nitrogen fertilizer on the pasture yield and composition, diet selection by cows, and soil fertility of Gatton panic (Panicum maximum cv. Gatton) pastures. Thirty-two Friesian cows were used in a 4x2 factorial design: four stocking rates (2.0, 2.5, 3.0 and 3.5 cows/ha), each at two rates of fertilizer application 200 and 400 kg N/ha.year. The higher rate of fertilization increased the pasture green dry matter on offer at all samplings (P < 0.01); the increase ranged from 1 106 kg/ha in summer to 548 kg/ha in spring. Green dry matter decreased ( P< 0.0 1) with increasing stocking rate, with mean yields of 3736 and 2384 kg/ha at 2.0 and 3.5 cows/ha, respectively. Weed yields increased over the 3 years at the higher stocking rates for pastures receiving 200 kg N/ha.year. The crude protein content of leaf and stem increased with increasing stocking rate and amount of applied nitrogen fertilizer. Values ranged from 12.1 to 26.5% of dry matter (DM) in leaf and from 3.7 to 13.8% DM in stem. In leaf, sodium concentration (range 0.05-0.20% DM) was increased, while phosphorus concentration (range 0.21-0.44% DM) was decreased by the higher rate of fertilizer application. Plant sodium and phosphorus levels were inadequate for high levels of milk production. Dietary leaf content and crude protein contents were consistently increased by both a reduced stocking rate, and the higher rate of fertilization. Cows were able to select for leaf and at the lowest stocking rate, leaf in the diet averaged 38%; while the leaf content of the pasture was 20%. Dietary leaf content ranged from 38 to 57% in summer and from 11 to 36% in winter. Dietary crude protein ranged from 13 to 15% in summer and from 7 to 11% in winter and was positively correlated with pasture crude protein content and dietary leaf percentage. Soil pH decreased (P<0.05) from an overall mean of 6.3 in 1976 to 6.1 at 200 N and 5.8 at 400 N in 1979. Soil phosphorus status remained stable, while calcium and magnesium levels were lower (P<0.01) after 3 years.
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