To achieve the targeted artificial insemination coverage with the current rate of semen production, without affecting the conception rate, it needs to reduce the number of spermatozoa per insemination dose in India as per international practice. Therefore, this study was planned to perform different levels of semen dilution, compare in vitro post-thaw semen quality and develop a modified low-dose semen packaging method in French mini straw to minimise semen dilution effect. Sixteen ejaculates were collected from Karan Fries bulls (n = 4). The mean percentage post-thaw motility, viability, membrane integrity, acrosome integrity, lipid peroxidation and capacitation status were estimated as post-thaw sperm function assays in semen sample diluted to 20, 15, 10 and 5 million spermatozoa per 0.25 ml and filled in the French mini straw by conventional packaging. No significant (p > .05) difference in post-thaw sperm quality was observed between 15 and 20 million doses; however, below 15 million sperm quality get reduced. There was no significant difference in post-thaw semen quality traits between 20 million conventional packaging and 5 million spermatozoa/dose in modified packaging. In conclusions, the modified packaging is a very effective method for low-dose cryopreservation with acceptable post-thaw semen quality.

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Background: The Indian dairy industry has progress consistently ever since the White revolution of the 1970s, making India, the world’s largest and fastest producer of milk with 17 per cent global share. The Indian dairy market is expected to double within the next 10 years, primarily driven by over 16-20 per cent growth in value added dairy segment. To catch this high growth potential and to meet the rising demand, a sustainable and strong dairy production system will be critical. Methods: A study was conducted between December 2018 and February 2019 at four different dairy farms. The farms were identified based on rearing systems practiced. The farms were divided into two groups where the first one (n=10 dairy cattle) utilized automatic rearing systems (the ARS farms), while the second group (n=10) had conventional rearing systems (the CRS farms).Result: Based on the results, the effect of different rearing systems on the average lactation yield in the fourth lactation was significantly higher (P≤0.05) in automatic rearing system. The lactation yield of both the treatment groups was not significant till third lactation. There was no significant difference observed in persistency of milk production in both the rearing systems. Reproductive performance of the ARS houses had better age at first calving and service period as compared to conventional house type with significant difference. By using an ARS it is possible to save time and achieve greater flexibility. The experiment indicates less man power minutes required for routine daily work like feeding, watering and milking in automatic rearing system as compare to conventional rearing system. A significant (P≤0.01) reduction in working time by comparison with a different feeding, watering and management system however can only be expected in the case of sizeable herds. It appears that not much time can be saved with herds numbering 60 animals, but flexibility for the farm manager becomes significantly greater. In view of the relatively high amount invested in ARS, the profitability of such a system must be decided on a farm by farm basis. In principle an ARS can be a good opportunity for optimizing working time and workload in dairy farming.

Background: The productivity and profitability of commercial broiler largely depend on nutrition and growth performance and, therefore, this industry requires high quality protein sources, such as Corn Gluten meal (CGM). Many studies have been conducted on protein quality changes and other nutritional characteristics of CGM in several countries Corn gluten meal contains around 60% CP and is a byproduct of wet milling of corn, most of which is used for manufacture of high fructose corn syrup and corn flour. Being high in crude protein, it is often compared to animal protein ingredients during feed formulation. Thus, this study was intended to evaluate the effect of feeding corn gluten meal in feed ration on growth performance of commercial broiler chicken. Methods: A feeding trial was conducted in the year 2018 with an objective to assess the effect of feeding corn gluten meals (CGM) in broiler chicken. Six hundred broiler chickens (day old) were randomly allotted to three groups, comprising of fifty broiler chickens in each treatment group with four replicates. The group-I (T1) Broiler chicken were fed with composite diet containing 5% Corn Gluten meal where as Group-II (T2) received composite diets comprising of CGM 10%. The Group-III (T3) served as control group with normal broiler diet. The experimental period lasted for six weeks. Weekly body weight and feed consumption were measured in each group. Result: Diet composite of 10% of corn gluten meal had significantly (P≤0.05) higher weight gain, lower feed intake and improved FCR than T1 and T3 group. CGM supplementation can be ideally improved broiler weight gain, feed consumption and FCR.

Background: Baseline information on the morphological development of laboratory animals is very scanty. Hence the present study was undertaken to understand the morphological development of experimental animals. Methods: An experiment was conducted at Biogen animal facility, Bangalore in the year 2018 to study the morphological changes with regard to growth and developmental parameters in Swiss albino mice and Wistar rats and body weight in guinea pigs. Ten Swiss albino mice and Wistar rats in advanced pregnancy of similar age groups and comparable litter sizes in the previous kindlings were selected for the experiment, where as five guinea pigs males and females each were selected with same age group. During the study period, litter weight and size at birth and at weaning, time of initiation and completion (full growth of hair) of hair growth, time of opening of eyes and ears recorded in Swiss albino mice and Wistar rats whereas body weight at birth, 3rd day, 6th day, 9th day, 12th day and 15th were recorded in Dunkin Hartley guinea pigs. Result: The results of Swiss albino mice indicated that the average litter weight (grams) and litter size at birth ranged from 1.39 and 7.50 respectively. The average time taken from initiation and full growth of hair covering on body was 7.50 to 15.30 days. Eyes and ears opened at 7.90 and 8.20 days respectively. Average weight of male and female recorded at weaning (25 days) 19.38 and 15.12 respectively and the litter size at the weaning was 9.70 whereas, livability percent was recorded 87.81 at the end of the trial. The results of Wistar rats indicated that the average litter weight (grams) and litter size at birth ranged from 5.07 and 11 respectively. The average time taken from initiation to full growth of hair covering on body was 9 to 16.20 days. Eyes and ears opened at 12.20 days, average weight (grams) of male and female recorded at weaning (25 days) 78.03 and 63.09 respectively. The litter size at the weaning was 9.70. The livability percent was recorded 88.02 at the end of the trial. The results of Dunkin Hartley guinea pigs indicated that the average body weight (grams) of female at birth, 3rd day, 6th day, 9th day, 12th day, 15th (Weaning period) ranged from 94.88, 109.34, 123.94, 139.74, 152.14 and 166.66, respectively. On the other hand, average body weight (grams) of male at birth, 3rd day, 6th day, 9th day, 12th day, 15th (Weaning period) ranged from 145.38, 155.42, 170.50, 185.54, 200.64 and 215.7, respectively.