Background: In COVID scenario World Health Organization (WHO) recommends washing hands with water and soap or alcohol-based hand rubs for cleaning hands. Hand hygiene has been considered a central component in every program related to infection control.  It is applicable to healthcare personnel handling critical patients as well. Poor hand hygiene during intrapartum and newborn care is associated with increased death rates among newborns due to sepsis. This study aims to assess the knowledge, attitude, and practices of handwashing by healthcare staff in the hospital. Methodology: This is an observational study which is conducted among the healthcare personnel at AVBRH hospital, Wardha. A tool consisting of a self-administered observation record form on knowledge and practices of handwashing will be used for data collection from 60 healthcare workers. Data will be analyzed using appropriate statistical tests. Expected Results: Appropriate levels of knowledge and practices about proper hand hygiene are expected within healthcare workers of AVBRH.  Availability of alcohol-based hand rubs, sanitizers, soap and water is expected at all times. Conclusion: Considering the pandemic situation, the availability and use of hand sanitizers can be effective to limit the spread of infections COVID -19.