The present uses of seaweed Kappaphycus alvarezii are as human foods, cosmetics, fertilizers, and for the extraction of industrial gums, chemicals and most importantly seaweeds are rich in source of Omega-3 fatty acid. The aim of present study involves preparation of poly herbal hair oil using methanol extract of brown seaweed and the other herbal plants. The prepared herbal oil was subjected to phytochemical screening, antioxidant activity, antibacterial activity, general characterization, physical and biological evaluation. The current studym involves preparation of poly herbal hair oil using Kappaphycus alvarezii, Aloe barbadensis miller, Murrays Koenigii and Ocimum tenuiflorum. The oil was prepared according to Ayurvedic pharmacopeia. The prepared formulations are assessed for primary skin irritation test on our forearm. The above said parameters were found to be good and within the standards. All the values in the evaluation of finished K. alvarezii based herbal oil showed that they are within the acceptable limits. It is a very good attempt to establish the herbal hair oil containing methanol extracts of red seaweed and the other herbal plants. Hence, it is concluded that the oil is beneficial in maintaining good growth of hairs, turning grey hairs to black, providing protection from dandruff, and results in lustrous looking hairs.

The present study was to determine the phytochemical constituents, antioxidant, and anticancer potential of methanolic extract from the seeds of Trachyspermumammi on KB, human oral cancer cell line using in the vitro assay system. The seeds of the T. Ammi were extracted with methanol. Phytochemical screening was done using standard qualitative biochemical tests. The antioxidant activity of the T. Ammi extract was determined by using a phosphomolybdenum assay.MTT assay was used to estimate the antiproliferative activity of the extract against the human oral cancer (KB) cell line. The apoptotic inducing ability of the extract was investigated by intracellular ROS analysis, antioxidant enzyme levels (SOD, CAT, GPx, and GSH), EtBr/AO staining, and TBARS analysis. The qualitative phytochemical analysis revealed the presence of different secondary metabolites. The phytochemical content present in the methanolic extract possessed high antioxidant capacity with transition metal reduction in phosphomolybdate assay. The methanol seed extract was found to be cytotoxic in vitro to (KB) oral cancer cell lines with IC50 value125µg/ml. Significantly, the T. Ammi seed extract showed a broad-spectrum anti-cancer activity through promoting ROS generation via modulating apoptosis induction in KBoral cancer cells. From the results, it can be concluded that T. ammiseed extract was a promising antioxidant and anticancer agent for KB human oral cancer cell line. The results figure the sufficient scientific backgrounds to isolate and purify the bioactive compounds in future for further applications.