A new simple, precise, accurate and selective TLC-densitometry method has been developed for simultaneous determination of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form. Chromatographic separation was performed on aluminum plate precoated with silica gel 60F254, using chloroform: ethanol: glacial acetic acid fumarate and hydrochlorothiazide were 0.62 and 0.40, respectively. The reliability of the method was assessed by evaluation of linearity which was found to be 200-1200 ng/spot for bisoprolol fumarate and 100-800 ng/spot for hydrochlorothiazide. Accuracy of the method was accessed by % recovery and found to be 100.02 ± 1.14% for bisoprolol fumarate and 99.91 ± 0.96% for hydrochlorothiazide. The method can be used for routine analysis of bisoprolol fumarate and hydrochlorothiazide in tablet dosage form.

A simple, precise, rapid, selective, and economic reversed phase high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of Ambroxol Hydrochloride and Doxofylline. The HPTLC method was performed on precoated silica gel G60 F254 plates with Diethyl ether:n-butanol:Ammonia (9:0.9:0.1 v/v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their absorption at approximately 254 nm for AMB and DOX. The drugs were satisfactorily resolved with R f 0.29 ± 0.02 for and R f 0.56 ± 0.02 for DOX. The calibration plot was linear between 100-600 ng /band for AMB and 200-1200 ng/band based for DOX. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined dosage form.