Bacteria found in blood circulation either consistently intermittently are commination to every organs of body. These infections can affect life and death. In India also blood stream infections are major causes of health problem that caused demise of patients in hospital. Timely diagnoses of infection with antimicrobial susceptibility assessment are important for optimization of treatment and best way to reduce hospital stay and improve patient health. In spite of recent advances in clinical diagnostics, blood culture remains the gold standard for the detection of blood stream infections. Studies in different places and regions have indicated the varying microbiological pattern of bloodstream infections which support the need for a continuous examination of the causative organisms. For the diagnosis of septicemia, Blood cultures are the “gold standard” are based on the detection of viable microorganisms in the blood. The main aim of this is to identify the bacteria causing bloodstream infections and to determine and analysis their antibiotic sensitivity pattern in a tertiary center. In this study blood for culture was collected from 940 clinically suspected cases of blood stream infection from the hospital. Collected blood samples were processed in the bacteriology section at microbiology laboratory and standard laboratory methods were used to identified isolates and then antibiotic susceptibility test was performed using CLSI guidelines. Total 940 blood samples were cultured in which 139(14.8%) were found positive. Among isolates, the most predominant organism was Staphylococcus aureus (51.8%) followed by Escherichia coli (24.5%) and the least was Salmonella species (1.4%), Proteus species (1.4%) and Acinetobacter species (1.4%). Among Gram positive isolates, Penicillin and Erythromycin showed high degree of resistance. Imipenem was particularly susceptible among the isolated. Gentamicin and Amikacin showed high in vitro susceptibility for both Enterobacteriaceae and Nonfermenters. This study provides information on bacteriological profile of septicemic isolates. Therefore continuous monitoring of the susceptibility of organisms towards antibiotics is necessary to prevent and spread of drug resistance.

Introduction: Polycystic ovary syndrome(PCOS) is a common endocrinal disorder of reproductive age, that cause enlarged ovaries with small cyst on the outer edges with infrequent or prolonged menstrual cycles, excess hair growth, acne and obesity, which is nowadays prevailing among females at adolescent stage. About 5-10% of women among the general population of in the world are affected by PCOS. There is an increase in the prevalence of PCOS among the women and young girls of reproductive age, where they experience depression because of PCOS particularly. The exact cause of PCOS is unknown. The early diagnosis and treatment along with weight loss may reduce the long term complications of PCOS. It is not only a problem associated with reproduction, but also has associated vital metabolic and psychological health risks. Aim: Depression Due to Polycystic Ovary Syndrome in Adolescents Conclusion: In comparison to safe controls, adolescents with PCOS had lower self-esteem, more anxiety, and more depressive symptoms. Future research is required to look into the psychiatric issues that affect adolescents with PCOS.

Introduction: The diagnosis of extrapulmonary tuberculosis (EPTB) is a challenge. World Health Organization (WHO) recommends the use of GeneXpert MTB/RIF assay [Cepheid, United States of America (USA)], using a heminested real-time polymerase chain reaction (PCR) to amplify a Mycobacterium tuberculosis-specific sequence of the rpoB gene for the rapid and simultaneous detection of M. tb complex (MTBC) and resistance to rifampicin from a clinical specimen. The purpose of this study is to evaluate the performance of the GeneXpert MTB/RIF test with conventional mycobacterial culture in EPTB specimens. Materials and methods: This prospective study (February–October 2017, 11 months) includes data on 287 EPTB specimens that were processed by conventional culture on Lowenstein–Jensen (LJ) medium and the rapid molecular-based GeneXpert MTB/RIF assay system. Results: Among the 287 EPTB samples tested, GeneXpert detected the deoxyribonucleic acid of MTBC in 51 samples (17.8%). Standard bacteriological assays, including acid-fast bacilli microscopy and culture, were positive in 26 (9.1%) and 35 (12.1%) specimens respectively. The performance of GeneXpert results was evaluated against culture as a gold standard. The overall sensitivity and specificity of the Xpert assay were calculated to be 94.6 and 94.4%, respectively. The sensitivity of the Xpert assay with tissue specimens was 84.6 and 80.7% specificity, while there was 86.6% sensitivity and 98.1% specificity with the body fluids. Conclusion: GeneXpert had high performance than culture for the EPTB specimen. It can be a useful tool for the early diagnosis of patients with high clinical suspicion of EPTB. The other major advantage of GeneXpert is that it simultaneously detects rifampicin resistance within 2 hours.