Bioenhancers are drug facilitator which do not show the typical drug activity but in combination to enhance the activity of other molecule in several way including increase the bioavailability of drug across the membrane, potentiating the drug molecules by conformational interaction, acting as receptor for drug molecules and making target cell more receptive to drugs and promote and increase the bioactivity or bioavailability or the uptake of drugs in combination therapy. The objective of the present study was to evaluate the antibacterial and activity of combination in Azadirachta indica extract with cow urine distillate and pepper extract against common pathogenic bacteria, a causative agent of watery diarrhea. It has been found that Indian indigenous cow urine and its distillate also possess bioenhancing ability. Bioenhancing role of cow urine distillate (CUD) and pepper extract was investigated on antibacterial activity of ethanol extract of Azadirachta indica. Antibacterial activity of ethanol extract neem alone and in combination with CUD and pepper extract were determined the ATCC strains against Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and E-coli by cup plate diffusion method. Ethanol extract of neem has showed more effect on P. aeruginosa, E-coli than S. aureus and K. pneumonia with combination of CUD and pepper extract. CUD and pepper did not show any inhibition of test bacteria in low concentration. The antibacterial effect of combination of extract and CUD was higher than the inhibition caused by extract alone and is suggestive of the bioenhancing role of cow urine distillate and pepper. Moreover, inhibition of test bacteria was observed with less concentration of extract on combining with CUD

Alopecia is common and most frequently complaint dermatological disorder since the time immemorial. Various herbal and allopathic formulations are reported to have hair growth promoting activity. Minoxidil and Finasteride are only US FDA approved drugs for alopecia and hair loss. Maharanga bicolor and Hibiscus rosa sinensis are two ethnomedicinal herbs commonly used for hair growth promotion. Present study was conducted to determine hair growth promoting activity of petroleum ether extract of M. bicolor and H. rosa sinensis in Aloe vera gel. Minoxidil Sulphate and Different formulations of petroleum ether extract of M. bicolor and H. rosa sinensis was formulated in Aloe vera gel and applied to denuated area of rats for 30 days. Hair growth pattern, hair growth initiation, hair growth completion and length of hair was measured. M. bicolor (2%) formulation shows shortest hair growth initiation time on 6 th day compared to 11 th day in blank sample. Formulation containing 2% of each extract have shortest hair growth completion time on 19 th day of sample application and showed potent hair growth pattern observed by blind investigation. The result suggests that petroleum ether extract of M. bicolor and H. rosa sinensis possess good hair growth promoting activity.