UV-C processing of whole milk (WM) using a designed pilot scale Dean flow system was conducted at flow rates (11.88, 23.77, and 47.55 gph), Reynolds number ranges from 2890-11562 and the Dean number (at curved region) calculated as (648-2595) to inactivate bacterial endospores and virus particles. Biodosimetry studies were conducted to quantify the reduction equivalent fluence at selected experimental conditions. Results revealed that the dose distribution improved as flow rate increases, attributed to increase in Dean effects and turbulence intensity. Microbial inactivation studies conducted at 47.55 gph showed 0.91 (stdev:0.15) and 2.14 (stdev:0.19) log reduction/ pass for B. cereus endospores and T1UV phage. Linear inactivation trend was observed against number of passes which clearly demonstrates equivalent dose delivery during each pass. Lipid peroxidation value and volatile profile did not change significantly at UV dose of 60 mJ/cm 2. Lower E EO value signifies the higher electrical efficiency of the system.
A novel continuous thin-film (1.59 mm) serpentine path coiled tube (SPCT) UV system operating at 254 nm wavelength was designed and compared with flow field distribution of whole milk with helical path coiled tube (HPCT) UV system using computational fluid dynamics. The results revealed efficient velocity magnitude distribution at serpentine bend geometric locations of the SPCT UV system. Further in this study, we evaluated B. cereus Spores inactivation in whole milk (WM) and almond milk (AM) using the developed SPCT UV system. Experimental data showed that > 4 log reduction of spores was achieved after six and ten passes of WM and AM at a flow rate of 70 and 162 mL/min, respectively. The bio-dosimetry method was used to verify the delivered reduction equivalent fluence (REF) and reported as 33 and 36.5 mJ/cm2. We noticed no significant effect on lipid oxidation and volatiles profile (p > 0.05) up to delivered REF of 60 mJ/cm2. This study demonstrated that high levels of inactivation of B. cereus spores could be feasible with minimal impact on product quality by UV-C processing of dairy and non-dairy opaque scattering fluids.
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