In recent years, the development of RNA-guided genome editing (CRISPR-Cas9 technology) has revolutionized plant genome editing. Under nutrient deficiency conditions, different transcription factors and regulatory gene networks work together to maintain nutrient homeostasis. Improvement in the use efficiency of nitrogen (N), phosphorus (P) and potassium (K) is essential to ensure sustainable yield with enhanced quality and tolerance to stresses. This review outlines potential targets suitable for genome editing for understanding and improving nutrient use (NtUE) efficiency and nutrient stress tolerance. The different genome editing strategies for employing crucial negative and positive regulators are also described. Negative regulators of nutrient signalling are the potential targets for genome editing, that may improve nutrient uptake and stress signalling under resource-poor conditions. The promoter engineering by CRISPR/dead (d) Cas9 (dCas9) cytosine and adenine base editing and prime editing is a successful strategy to generate precise changes. CRISPR/dCas9 system also offers the added advantage of exploiting transcriptional activators/repressors for overexpression of genes of interest in a targeted manner. CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) are variants of CRISPR in which a dCas9 dependent transcription activation or interference is achieved. dCas9-SunTag system can be employed to engineer targeted gene activation and DNA methylation in plants. The development of nutrient use efficient plants through CRISPR-Cas technology will enhance the pace of genetic improvement for nutrient stress tolerance of crops and improve the sustainability of agriculture.

Chloroplasts and photosynthesis are the physiologically fateful arenas of salinity stress. Morphological and anatomical alterations in the leaf tissue, ultrastructural changes in the chloroplast, compromise in the integrity of the three-layered chloroplast membrane system, and defects in the light and dark reactions during the osmotic, ionic, and oxidative phases of salt stress are conversed in detail to bring the salinity-mediated physiological alterations in the chloroplast on to a single platform. Chloroplasts of salt-tolerant plants have evolved highly regulated salt-responsive pathways. Thylakoid membrane remodeling, ion homeostasis, osmoprotection, upregulation of chloroplast membrane and stromal proteins, chloroplast ROS scavenging, efficient retrograde signalling, and differential gene and metabolite abundance are the key attributes of optimal photosynthesis in tolerant species. This review throws light into the comparative mechanism of chloroplast and photosynthetic response to salinity in sensitive and tolerant plant species. Highlights• Photosynthesis is highly sensitive to salinity stress • An interplay of physiological and molecular determinants exists in the sensitivity of chloroplast to salinity stress • The major tolerance and adaptive mechanisms to salinity are chloroplast membrane remodeling, ion homeostasis, and retrograde signalling

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