Latar Belakang. TGF-β merupakan growth factor yang paling dominan dalam peningkatan sintesis kolagen, memiliki peran utama pada penyembuhan luka dengan menstimulasi fibroblas sehingga menimbulkan penyembuhan dan berperan serta dalam pembentukan parut, baik itu parut normal maupun abnormal seperti parut hipertrofik dan keloid. Penelitian ini bertujuan untuk mengukur kadar TGF-β pada fase penyembuhan luka.Metode. Penelitian eksperimental ini menggunakan randomized post test only control group design. Dua belas luka akut kulit tikus dirandomisasi menjadi dua kelompok, dimana kelompok 1 diambil spesimen pada hari ke-5 dan kelompok 2 pada hari ke-21 dan dilakukan pemeriksaan ELISA untuk mengukur kadar TGF-.Hasil. Pengukuran kadar TGF-β pada luka akut kulit tikus didapatkan jumlah yang meningkat secara signifikan dari hari ke-5 (fase inflamasi) ke hari ke-21 (fase proliferasi) dengan nilai p = 0,003. Kesimpulan. Terjadi peningkatan kadar TGF- pada akhir fase proliferasi atau awal fase remodelling. Hal ini menyebabkan peningkatan proliferasi fibroblas untuk mensintesis kolagen yang nantinya dapat menjadi parut hipertrofik dan keloid.

Background: Bilateral cheilorraphy has higher complexity compared to unilateral cheilorraphy. Anthropometric measurements provide nasolabial identification of the surgery result objectively. This study aims to assess the outcome of bilateral cheilorrhaphy with the Djohansjah technique at Surabaya CLP Center.Methods: 26 patients underwent bilateral cheilorrhaphy from January 1 to December 31, 2018 at the Surabaya CLP Center. Anthropometric measurements were performed on control photos one year post-surgery with GIMP application (GNU Image Manipulation Software) version 2.10.12 (www.gimp.org) to measure the cupid’s bow width ratio, hemilabial length ratio, nasal width ratio, and philtrum ridge length ratio. A statistical analysis was performed using Independent Sample t-Tes and Mann-Whitney Test.Results: An anthropometric size difference test based on preoperative condition was performed. No significant difference of the cupid’s bow width ratio, hemilabial length ratio, nasal width ratio, and philtrum ridge length ratio between the group of identical cleft lip and unidentical cleft lip with p > 0.05 was found. Likewise, there was no difference between the identical cleft alveolar and unidentical cleft alveolar, as well as the group with or without cleft palate.Conclusions: Symmetry of lips, nose, and philtrum was achieved with bilateral cheilorrhaphy with the Djohansjah technique at the Surabaya CLP Center and there was no significant difference in cupid's bow width ratio, hemilabial length ratio, nasal width ratio, and philtrum ridge length ratio on preoperative cleft condition.

Imbalance of collagen synthesis and degradation causes formation of abnormal scarring leading to hypertrophic scar or keloid. Previous studies have shown that applying antihistamines could reduce the level of dermal collagen. However, no data whether topical antihistamine could be used as preventative therapy for abnormal scar formation. The aim of this study was to assess whether the application of topical antihistamine in acute wounds could affect the level of transforming growth factor beta (TGF-β), a regulator of wound healing process, and collagen in wound healing phase in order to suppress the keloid formation. A randomized post-test only control group design study was conducted. Twenty-four acute wounds on Rattus novergicus rats were randomized divided into control and treatment groups. The animals of treatment group received daily topical antihistamine consisting 1% diphenhydramine hydrochloride and 0.1% zinc acetate. The full-thickness skin tissue from each rat were taken on day 5 and 21 and were subjected to ELISA test to measure the levels of TGF-β and collagen. Our data found that in the treatment group, the level of collagen decreased significantly over time (1623.61ng/mL on day 5 to 755.15ng/mL on day 21, p=0.001). Similarly, the level of TGF- also decreased although was not statistically significant (p=0.766). In contrast, the levels of TGF-β level and collagen in the control group increased significantly from day 5 to day 21, p=0.003 and p=0.001, respectively. The level of TGF- in treatment group was significantly higher compared to the control group p=0.001 on day 21 while the level of collagen had no different between treatment and control group (p=0.124). In conclusion, our data suggest that topical antihistamine reduced the levels of TGF-β and collagen at the end of the proliferation phase or the beginning of the remodeling phase. Further study is warrant to elucidate the effect of antihistamine on other would healing markers or parameters.