As heavy metals are bioaccumulative and toxic in nature, increasing heavy metal levels in the environment is of great concern. The present study was aimed to assess the acute toxicity effects of copper on some behavioral attributes of Filopaludina bengalensis (locally known as ‘Genri’), a freshwater gastropod, under laboratory condition. Exposure to copper (0.50-2.0 ppm) for 96 h resulted in significant mortality. Recorded LC50 value at 96h was 0.586 ppm. The aggregation tendency, crawling activity and mucus secretion were observed at different exposure period upon acute exposure of copper (0.586 ppm).With the advancement of exposure period, gradual deaggregation was observed in snails of copper treated sets. There was statistically significant difference (P <0.01) in aggregation response and foot protrusion response at different span of exposure in copper treated groups. In copper treated sets, a substantial increase (P<0.01) in mucus secretion (%) was evident with time. Foot protrusion and movement was inhibited in copper treated groups. All three responses in the copper treated groups were significantly different (P <0.01) from that of control groups. The behavioral responses observed in this study of Filopaludina bengalensis could be used as biomarkers for copper contamination in any aquatic ecosystems.

The Mundeswari River is the western distributary of the Damodar River system in eastern India. The water of this river is extensively used for domestic purposes and agricultural irrigation. This study aimed to evaluate the spatial and temporal water quality variation of the Mundeswari River and assess the water quality status of this river using the Canadian Council of Ministers of the Environment Water Quality Index (CCME-WQI). Water quality was monitored monthly at four selected sampling stations (M1, M2, M3, and M4) during 2020-2022, considering twelve selected water quality indicators. The obtained water quality data were analysed using different statistical techniques. Water quality at different monitoring stations was appraised through the use of CCME-WQI. The results revealed that the overall water quality of most of the monitoring stations based on CCME-WQI values was “marginal.” The highest WQI value (82.01) was observed at M1, and the lowest WQI (41.24) was recorded at M3. One-way ANOVA indicated a statistically significant difference in WQI values between sampling sites (P <0.05). The water quality of the M3 sampling station was found to be in degraded condition throughout the study period. Cluster analysis from the perspective of WQI values revealed two distinct clusters of the sampling stations. Substantial seasonal variation in water quality was also observed. This river had putrid water quality during the pre-monsoon period, and relatively better water quality was evident after the monsoon. This study revealed that the water of the Mundeswari River is utterly unsafe for human consumption and it requires significant treatments before it can be safely used for domestic purposes like cooking, washing etc.

This study aims to assess the acute toxicity of commercial-grade Cypermethrin (10% EC) and evaluate the hematological and behavioral alterations in a freshwater fish Channa punctatus upon short-term exposure to Cypermethrin. A four-day static acute toxicity test was performed to estimate the median lethal concentration (LC50) value of Cypermethrin. During the acute toxicity test, the behavior of the control and cypermethrin exposed fish was critically observed and recorded. After completing the acute toxicity test, the hematological effects of Cypermethrin in C. punctatus were evaluated using two sublethal dosages (0.08 mg/L and 0.12 mg/L). Results of the study revealed that this pesticide induced significant mortality in C. punctatus with a 96-h L50 value of 0.263 mg/L. Cypermethrin exposed fish showed hyperactivity, irritability, erratic swimming, frequent surface visit, etc. Exposure to sublethal concentrations of Cypermethrin for a short period resulted in a significant decline (P<0.05) in total erythrocytes count (TEC), packed cell volume (PCV), mean corpuscular volume (MCV), and hemoglobin (Hb) concentration as compared to control groups. In contrast, pesticide-exposed groups had a significant increase (P<0.05) in mean corpuscular hemoglobin concentration (MCHC) and total leucocyte count (TLC). It is apparent from the results of the study that this commercial formulation is toxic to the studied fish. This study also revealed hematological and behavioral alterations in C. Punctatus which could be used as biomarkers for incipient Cypermethrin intoxication.