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Fusarium oxysporum f. sp. lycopersici (FOL) is one of the utmost complex and adaptive species in the Eumycota, it is a soil borne pathogen of a wide range horticultural and food crops causing wilts, rots and damping diseases (Bodah, 2017). Of particular, interest is FOL significant contribution to tomatoes vascular wilt disease. Solanum lycopersicum L. of the family Solanaceae and commonly known as tomato is a heavily consumed crop throughout the world and important source of vitamins A and C (Hanson et al., 2016). Serious agricultural loss before and during harvest and decrease in economic value of tomatoes are often caused by the vascular wilt due to FOL. Tomato vascular wilt is the most destructive and devastating disease characterized with invasion of the xylem vessels from the root thereby leading to wilting and death of the plant. (Swarupa et al., 2014). FOL often harbour secreted in xylem (SIX) and tomatinase (TOM) proteins during its growth which provide support to its pathogenicity against this crop (Sonkar & Pritam, 2020). The management of Fusarium sp in crop production is often by the use of expensive fungicide (de la Isla & Macías-Sánchez, 2017),

The present investigation was carried out on identification and characterization of an antagonism band of extract secondary metabolites from Trichoderma asperellum MK045610 against Fusarium oxysporum f. sp. ciceri and Fusarium oxysporum f. sp. lycopersici. In this experiment, analysis was performed by High Performance Thin Layer Chromatography (HPTLC), paper disc assay, gas chromatography-Mass spectrometry (GC-MS). Firstly, extract crude secondary metabolites were used for partial purification based on HPTLC. Secondly, the paper disc assay method was used for the determination of antifungal property on PDA plate from the partial purified compound. Thirdly, GC-MS was used for identification of partial purified compound based on peaks. Identified compounds are named as Phenol, 3, 5-bis (1,1-dimethylethyl), Pentadecanoic Acid, 14-methyl, methyl ester, Benzenepropanoic acid, 3,5,-bis (1,1-dimethyl ethyl)-4-hydroxy-methyl ester and represented to antifungal property . Conclusively, Secondary metabolite of Trichoderma asperellum MK045610 has a significant role in radial growth inhibition of Fusarium oxysporum f. sp. ciceri and Fusarium oxysporum f. sp. lycopersici.

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