The purpose of this research was to extract tea leaf phenols using Microwave Assisted Extraction (MAE) method at 3 levels of microwave power (high, medium high and medium) and investigated the influence of physco chemical properties of whey protein and gelatine biopolymer using tea leaf extract as crosslink materials at different concentration (5%, 10% and 15% (v/v)). MAE method gave significantly effect on phenolic content. High level power of MAE gave higher phenolic content of tea leaves extracts. Tea leaves extracts as crosslinked agent of biopolymer gave highly significant effect on the stability of the emulsion, the emulsion activity and foaming power. SDS-PAGE protein profile showed increase molecular weight with the addition of tea leaf extract, it can be presumed presence crosslinked both on whey protein or gelatine.

Gembili is one variety of local tubers originating in Indonesia, where its utilization is not in its most use. This tuber has bioactive compounds such as dioscorin, diosgenin, inulin and water-soluble polysaccharides that function as immunomodulators, including those which prevents metabolic diseases such as hyperglycaemia. The addition of gembili tubers in fermented milk has more value and is an alternative nutraceutical food for people who need a healthy diet because it contains probiotics and bioactive components. This study aims to determine the antihyperglycemic activity of fermented milk enriched with gembili flour in animal models. Probiotics contained in fermented milk are Lactobacillus acidophilus, Lactobacillus plantarum and Streptococcus thermophilus. The experimental animals used in this study are healthy male white rats, not deformed, and had never been used in previous experiments. The animals in experiment were grouped into five treatment groups namely P1=negative control, P2=positive control, P3=1 ml/rat/day of fermented milk with the addition of gembili tubers, P4=2 ml/rat/day of fermented milk with the addition of gembili tubers, and P5=3 ml/rat/day of fermented milk with the addition of gembili tubers (P5). The results of this study prove that the addition of fermented milk enriched with gembili showed significant results (p<0.05) on the decrease in blood sugar levels with the highest decrease of 43.59% in the P5 group.

Edible film is potentially applied to replace non-biodegradable packaging for animal products, e.g. meat and meat products. The overall objective of this study was to observe the effect of different addition levels of modified casein hydrolysate (casein-catechin complexes) on mechanical (film thickness, water vapor permeability, tensile strength, elasticity), chemical (moisture content, water activity, and solubility) and microstructure properties of composite edible casein film. The edible films were formulated according to different combinations of modified casein hydrolysate and casein film solution (0.50:0.25, 0.50:0.50, 0.75:0.25, and 0.75:0.50) and compared with control (without addition of modified casein). No significant effects of the treatment were found on film thickness, water vapor permeability, tensile strength, elasticity, moisture content, water activity, and solubility of the composite edible film. However, the micrographs show that the amount of polymer was higher as the proportion of catechin was increased. In conclusion, the addition of catechin-added casein (modified casein) to casein edible film solution at up to 0.5% did not alter the mechanical properties, moisture content, water activity and solubility of the composite edible film.

Meat is one of perishable foods that should be handled hygienically to preserve its quality. Edible coating containing antibacterial agent can be used for preserving meat by providing a barrier against microbial contamination. The objective of this study was to compare the effectiveness of edible coating made from casein-chitosan (CC) and casein-chitosan modified (CCM) on preserving the quality of meat during accelerated storage test. The coating solutions were characterized in terms of their composition (carbohydrate, protein, fat, moisture content, pH, Aw, and antioxidant capacity) in order to optimize coatings composition, then applied to the meat for accelerated storage test for 24, 120, 216, and 312 h at 7°C. The carbohydrate, protein, fat, ash, moisture content, pH, aw, and antioxidant capacity of CCM edible coating were different with those of CC edible coating. CCM edible coating showed better antioxidant effect than CC but showed comparable antimicrobial capacity in meat during accelerated storage. Therefore, CCM edible coating could be used to preserve meat.

This study aimed to determine the best technique of application of edible coatings (spraying, spreading, or dipping) on beef carried out sensory quality, then performed in coated to physicochemical analyses (pH, moisture content, WHC, cooking loss, and color). This study aimed to determine the best technique of application of edible coatings (spraying, spreading, or dipping) on beef carried out sensory quality, then performed in coated to physicochemical analyses (pH, moisture content, WHC, cooking loss, and color). The beef was coated with different coating techniques spraying, brushing, and dipping then stored at 27°C for 8 hours. At the end of the storage period, the treatments exhibited the best sensory analyses (texture surface and under, odor, physical deviation, and discoloration). The best sensory analysis is the spraying of coatings technique is spraying. The results that the application of the edible coatings technique on beef maintaining the best physicochemical quality are pH 5,59; the moisture content of 68,26%; WHC of 43,71%; cooking loss of 34,28%; and color indicated by L*, a*, and b*.