Aim: The present study was undertaken to analyze genetic diversity among pearl millet genotypes based on drought linked morpho-physiological and microsatellite markers. Study Design: In the present investigation, 96 pearl millet germplasm lines were screened against drought using different morphological and physiological traits along with SSR markers. Place and Duration of the Study: The present study was conducted at College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during July 2019 to December, 2020. Methodology: The study was conducted to record different morphological and physiological traits related to drought tolerance and susceptibility. Thirty five microsatellite markers were also used in laboratory to analyze the variability among pearl millet genotypes under study. Results: Pearl millet genotypes were grouped according to their morpho-physiological characteristics. Among 35 SSR markers, twenty-two were successfully amplified across all germplasm lines and seven SSR markers were found to be polymorphic and fifteen markers were monomorphic. All seven polymorphic SSR markers were used consequently for amplification of all the 96 germplasm lines. The range of PIC value was 0.0939 to 0.2980 with the average of 0.2274. The highest PIC value was recorded for the markers Xibmsp26 and Xibmsp29 (0.2980), followed by Xibmsp03 (0.2392), Xibmsp29 (0.2392), Xibmsp06 (0.2289) and Xibmsp07 (0.1948) while the lowest for the marker Xibmsp01 (0.0939). The range of major allele frequency value was 0.7604 to 0.9479 with the average of 0.8363. The range of genetic diversity value was 0.0987 to 0.3644 with the average of 0.2665. Conclusions: According to the morpho-physiological data a total of 22 pearl millet genotypes were found to be grouped distantly from rest of the genotypes. These genotypes had shown their drought tolerance bahaviour however, rests of the genotypes were found to be susceptible against drought.

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Aim: The current investigation was commenced to investigate genetic miscellany among pearl millet genotypes based on diverse biochemical parameters interrelated to drought tolerance. Study Design: In investigation, 96 pearl millet germplasm lines were screened against drought using diverse biochemical traits. Place and Duration of the Study: The present study was conducted at College of Agriculture, Gwalior, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M.P., India during July 2019 to December, 2020. Methodology: Five biochemical parameters viz., chlorophyll content, carotenoid content, total soluble sugars, proline and protein were investigated for explanation of differences among 96 pearl millet germplasm lines in respect to drought tolerance. Results: Data of present investigation revealed the mean leaves chlorophyll at 30DAS was 2.90 mgg-1 with the range of 1.31-4.69 mgg-1, whereas chlorophyll at 60DAS was arrayed between 1.46-3.84 mgg-1with an average of 3.02 mgl-1. Carotenoid at 30DAS was ranging from 4.5-11.44 mgg-1 with an average value of 7.23 mgg-1, while carotenoid at 60DAS was recorded in range of 5.01 to 10.10 mgg-1with an average of 6.66 mgg-1. TSS was ranged between 1.10-2.20 mgg-1, proline 0.10 to 0.17 mg g-1 and protein content 9.2-16.60 mgg-1. Conclusions: According to the biochemical data a total of 16 pearl millet genotypes were found to be grouped distantly among all the genotypes. Possibility existed to be drought tolerance of these genotypes.

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Powdery mildew disease of oilseed mustard caused by Erysiphe cruciferarum is a primary reason of yield reduction not only in India but also throughout the world. Identification and cultivation of resistant mustard genotypes against powdery mildew is the only way to overcome this challenge. In the present investigation, we targeted to screen 75 Brassica genotypes against powdery mildew based on disease indexing under field conditions and gene-specific molecular markers. Disease reaction on both the cotyledonary and true leaves was screened with using a modified 0-9 scale score as well as with nineteen disease linked microsatellite markers. In disease indexing under field conditions, genotypes viz., L-4 and PC-5 were identified as immune, China and RP-9 were considered as highly resistant and GSC-7 and PC-6 were recognized as resistant whilst genotypes i.e., RB-50, Pusa Bold, WRR-10 and GSL-1 were accredited as moderately resistant. Molecular markers based UPGMA dendrogram classified Rohini, WRR-22, PC-6, PusaBold, China, WRR-8, GSL-1, WRR-7, RH-749, L-4 and RB-50 as highly resistant mustard genotypes. In addition, disease linked marker cnu_m616 had the highest polymorphic information content (0.75) with greatest ability to differentiate resistant genotypes from susceptible genotypes, may be employed directly in mustard breeding programmes in future.