Aim of this study was to optimize the production of Ligninolytic enzyme for degradation of complex pollutants present in pulp paper industrial effluent (PPIE). Two ligninolytic enzyme-producing bacterial strains were isolated from PPIE and identified as Bacillus paramycoides strain BL2 (MZ676667) and Micrococcus yunnanensis strains BL3 (MZ676668). The identified bacterial strain Bacillus paramycoides strain BL2 showed optimum production of LiP (4.30 U/ml), MnP (3.38 U/ml) at 72 hrs of incubation, while laccase (4.43 U/ml ) at 96 hrs of incubation. While, Micrococcus yunnanensis strains BL3 produced maximum LiP (3.98) and MnP (3.85 U/ml) at 96 hrs of incubation and maximum laccase (3.85 U/ml) at 72 hrs of incubation, pH 7-8, and temperatures of 30-35°C. Furthermore, in the presence of glucose (1.0%) and peptone (0.5%) as nutrient sources, the enzyme activity of consortium leads reduction of lignin (70%), colour (63% ) along with COD (71%) and BOD (58%). The pollutants detected in control i.e. 3.6-Dioxa-2,7-disilaoctane , 2-Heptnoic acid ,trimethylsilyl ester, 7-Methyldinaphtho [2,1-b,1’,2’-d] silole , Hexadeconoic acid, trimethylysilyl ester, Methyl1(Z)-3,3-dipheny.1-4-hexenoale, 2,6,10,14,18,22-Tetracosahexane,2,2-dimethylpropyl(2Z,6E)-10,11epoxy5,6 Dihyrostigmasterol, acetate were completely diminished. The toxicity of PPIE was reduced up to 75%. Hence, knowledge of this study will be very useful for industrial sector for treatment of complex wastewater.
