Nanotechnology is a branch of science and engineering dedicated to materials, having dimensions in the order of nanometer scale and it has been widely used for the development of more efficient technology. Nanoparticles offer many benefits to bulk particles such as increased surface-to-volume ratio, and increased magnetic properties. In recent years, nanotechnology has been embraced by industrial sectors due to its applications in the field of electronic storage systems, biotechnology, magnetic separation and pre concentration of target analytes, targeted drug delivery, and vehicles for gene and drug delivery. Over the year’s nanomaterials such as nanoparticles, nanoclusters, nanoreods, nanoshells, and nanocages have been continuously used and modified to enable their use as a diagnostic and therapeutic agent in biomedical applications. Thus, In this chapter, introduction to metal nanoparticles, synthesis (Chemical and green synthesis) and biomedical application silver nanoparticles are presented.