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This study aimed to evaluate the impact of thermal processing and soaking on the profiles and contents of phenolic compounds in brown beans, as well as their antioxidant activity. We evaluated the antioxidant activity of extracts and the contents of phenolic acids and flavonoids by high-performance liquid chromatography (HPLC). With the exception of chlorogenic acid, the contents of all of the other phenolic acids were increased by cooking. Kaempferol was only detected in samples treated by soaking followed by cooking. Catechin and kaempferol-3-glucoside were found in all of the brown bean extracts. Cooking, with or without soaking, caused significant increases in the concentrations of quercetin and quercetin-3-glucoside only. The effect of the heat treatment increased the antioxidant activity and the concentrations of the phenolic compounds evaluated.

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Phenolic compounds and antioxidant activity of two bean cultivars (Phaseolus vulgaris L.) submitted to cooking Compostos fenólicos e atividade antioxidante de duas cultivares de feijões (Phaseolus vulgaris L.) submetidos à cocção AbstractThe common bean (Phaseolus vulgaris L.) is a source of nutrients and contains phenolic compounds that act as antioxidants. The aim of the present study was to determine the phenolic compounds and tannins in two bean cultivars (Phaseolus vulgaris L.): the biofortified carioca bean (Pontal) and the common bean (commercial). The antioxidant activity of the phenolic compounds and their fractions was also measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) methods. The thermal processing decreased the phenolic compounds, tannins and the antioxidant activity of beans. The Pontal cultivar exhibited higher levels of phenolic compounds even after cooking. For cooked beans, higher antioxidant activity was observed in the commercial beans by the DPPH method. Regarding to the fractions, in general, lower values of antioxidant activity by DPPH were observed for beans after cooking, except for fraction 6 of the Pontal bean and fraction 3 of the commercial bean. For fraction 4 no significant differences were observed by the ABTS method for both cultivars after thermal processing. Keywords: Legumes; DPPH; ABTS; Thermal treatment; Pontal bean; Common bean. ResumoO feijão (Phaseolus vulgaris L.) é uma leguminosa que, além de ser fonte de nutrientes, possui compostos fenólicos que atuam como antioxidantes. O presente estudo teve por objetivo a determinação de compostos fenólicos e taninos de duas cultivares de feijão (Phaseolus vulgaris L.): carioca biofortificado (Pontal) e comum (comercial). Averiguou-se ainda a atividade antioxidante dos compostos fenólicos e de suas frações, por meio dos métodos DPPH e ABTS. O tratamento térmico diminuiu os teores de compostos fenólicos, taninos e a atividade antioxidante dos feijões. A cultivar Pontal apresentou maiores teores de compostos fenólicos, mesmo após a cocção. Para as amostras de feijão cozido, observou-se maior atividade antioxidante para o feijão comercial pelo método DPPH. Quanto às frações fenólicas, observou-se decréscimo nos valores de atividade antioxidante por DPPH após o cozimento dos feijões, exceto para a fração 6 da cultivar Pontal e para fração 3 do feijão comercial. Para a fração 4, em ambas cultivares não foram observadas diferenças significativas na atividade antioxidante pelo método ABTS após tratamento térmico. Palavras