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O objetivo da presente pesquisa foi avaliar o ganho de peso, perfil lipídico, glicemia de jejum, função hepática e avaliação histológica de vísceras de ratos wistar submetidos a dietas especiais esperando estabelecer uma análise comparativa entre a estévia e a sacarose, perfazendo os seguintes tratamentos: T1 (controle); T2 (sacarose 4%); T3 (estévia 2%); T4 (estévia 4%) e T5 (estévia 6%) por 45 dias. Não houve significância para ganho de peso, consumo médio da ração, níveis de triglicérides, colesterol total e VLDLc, uréia e creatinina, independente da dieta ingerida. Os animais dos tratamentos T3 e T4 apresentaram menores níveis de glicose. Os animais do T2 apresentaram valor médio inferior, para o HDLc, enquanto que no LDLc e na ALT, o valor médio encontrado foi superior aos demais. Os animais, dos tratamentos T1 e T5, apresentaram níveis médios de AST inferiores aos demais. As lâminas histológicas apresentaram uma camada de gordura nas vísceras (rins) dos animais do tratamento T2 e nos demais animais dos outros tratamentos essa característica não foi observada. Neste contexto, a estévia apresenta características benéficas para a saúde, principalmente quando comparada a sacarose, podendo substituir o açúcar. Palavras-chave: Perfil lipídico, função renal e hepática, ganho de peso, estévia DOI: 10.5433/1679 AbstractThe aim of this study was to evaluate weight gain, lipid profile, fasting glucose, liver function, and histological evaluation of the organs of wistar rats subjected to special diets hoping to establish a comparative analysis of stevia, and sucrose, comprising the following treatments: T1 (control), T2 (sucrose 4%), T3 (stevia 2%) and T4 (stevia 4%) and T5 (stevia 6%) for 45 days. There was no significant difference for weight gain, the average consumption of diet, levels of triglycerides, total cholesterol and VLDLc, urea and creatinine levels independent of dietary intake. The animals of T3 and T4 had lower glucose levels. The animals of T2 had a lower average value for HDLc, LDLc, while in the AST, the mean value was higher than others. Animals, treatments T1 and T5, showed average levels of ALT inferior to the others. The histological sections showed a layer of fat in the viscera (kidney) of animals of T2 and for the other animals from other treatments that feature was not observed. In this context, stevia has beneficial characteristics to health and can replace sugar.

Citric acid is an important organic acid, multifunctional with a wide array of uses. The objectives of this study were the isolation and selection strains of the genus Aspergillus, investigating the solubilization of phosphate of these isolates, verifying the expression rate of genes involved in the identification of isolates, and efficiency of citrate synthase in the citric acid production. To verify the mechanisms of the microorganisms in solubilizing phosphate into the medium in the citric acid production, Araxá rock phosphate was utilized. Further, citric acid was evaluated in two culture media, namely BD and SA. The amplification by the polymerase chain reaction was done by using primer ITS region and citrate synthase (P1/P2). The isolates of Aspergillus niger were efficient to solubilize phosphate. The isolate A. niger 00118 stood out in the solubilization with an increase as high as five times the amount of soluble phosphorus when compared with the control treatment. The results of citric acid production highlighted better influence conditions of the culture medium Sabouraud (SA) on its production. The P1/P2 primer was sensitive in distinguishing the isolates A. niger 00116, A. niger 00104, A. niger 00098 and A. niger 00118 in the analysis of citrate synthase enzyme genes.