Aim:To compare the antimicrobial efficacy of herbal irrigants neem, miswak, propolis with sodium hypochlorite using conventional needle irrigation and EndoVac irrigation system against Enterococcus faecalis. Materials and methods:A total of 120 extracted single-rooted mandibular premolar teeth were infected for 21 days with E. faecalis after instrumentation with ProTaper system. Before irrigation procedure, dentinal shavings were collected in 1 mL of sterile broth and incubated. The optical density of each broth was measured using digital colorimeter and initial readings were recorded. Samples were then divided into four groups of 30 teeth each -Group I: Sodium hypochlorite irrigation, group II: Neem irrigation, group III: Miswak irrigation, group IV: propolis irrigation. Each group was further divided into two subgroups -(a) EndoVac irrigation (b) conventional needle irrigation. After irrigation, dentinal shavings were collected and optical density recorded. The values were analyzed statistically with Student's t test and analysis of variance followed by Tukey's honest significant difference test; p-value < 0.05 was considered to be statistically significant. Results:The postirrigation optical densities in all the groups were significantly lower than preirrigation values. Sodium hypochlorite demonstrated better antimicrobial efficacy followed by propolis, neem, and miswak. Differences in optical density values for all irrigants are higher in EndoVac (p < 0.0001) compared with needle (p = 0.0009) group, but it failed to reach statistical significance. Conclusion:Sodium hypochlorite proved to be a better irrigant followed by propolis, neem, and miswak. EndoVac irrigation system was more effective for elimination of E. faecalis than needle irrigation group.