Simarouba species are well known medicinal plants used in traditional medicine as anti-inflammatory, anti-oxidant and anti-cancer remedies. It is also known for having anti-neoplastic, anti-viral, anti-diabetic, herbistatic and analgesic properties. However, information on traditional use in tumour treatment are limited. Simarouba glauca is one of the most popular species, which is a native tree of Central and South America, also found in countries like South Florida, Caribbean islands, Cuba, Mexico, Costa Rica, Bahamas, Jamaica, etc. It was introduced in India in 1960. Even though studies on the biological activities of this species are abundant, still there is lack of data on the cytotoxic activity of Simarouba glauca extracts on Ovarian cancer in the literature. In this study, we analysed the phytochemical composition and anti-oxidant activity of these extracts. Cytotoxic potential of these extracts was estimated on PA 1 Human Ovarian Cancer cell lines. The methanol fraction exhibited better potency as compared to acetone fraction. Both acetone and methanol extracts showed higher anti-oxidant capacities in contrast to other solvent extracts.