Lysinibacillus sp. RGS degrades sulfonated azo dye Reactive Orange 16 (RO16) efficiently. Superoxide dismutase and catalase activity were tested to study the response of Lysinibacillus sp. RGS to the oxidative stress generated by RO16. The results demonstrated that oxidative stress enzymes not only protect the cell from oxidative stress but also has a probable role in decolorization along with an involvement of oxidoreductive enzymes. Formation of three different metabolites after degradation of RO16 has been confirmed by GC-MS analysis. FTIR analysis verified the degradation of functional groups of RO16, and HPTLC confirmed the removal of auxochrome group from the RO16 after degradation. Toxicity studies confirmed the genotoxic, cytotoxic, and phytotoxic nature of RO16 and the formation of less toxic products after the treatment of Lysinibacillus sp. RGS. Therefore, Lysinibacillus sp. RGS has a better perspective of bioremediation for textile wastewater treatment.

Combinatorial adsorption–biodegradation treatment of textile wastewater provides a cost effective and ecofriendly alternative to conventional physicochemical treatment methods.

No abstract

Fusarium oxysporum kolhapuriensis, a novel endophytic fungi isolated from Nothapodytes nimmoniana Mabb. Grahm, was found to produce camptothecine (CPT) using whey as a complex medium. The highest production of CPT was attained using statistical methods Response Surface Methodology (RSM). Central Composite Design (CCD) was used to optimize the complex medium and culture conditions for maximum production of CPT by the fungus. The optimized medium that yielded 283 AE 0.27 mg l À1 of CPT contained 70% (v/v) of acid whey and 2% (w/v) malt extract. The other two culture parameters optimized through RSM were temperature (30 C) and period of incubation (6 days). The production of CPT was confirmed by analytical techniques such as HPTLC, HPLC and LC-HRMS. This cost effective optimized medium using RSM might be useful for large scale CPT production which will ultimately reduce the further downstream processing cost.