The review article deals with theoretical aspects of Derivative UV-Spectrophotometry. The method gains significance using the first and second derivative of the transmission spectra with respect to wavelength. Generated optical derivatives are compared to the known numerical derivatives. The derivative spectra from 1 st to 4 th are consequently discussed. This provides valuable insight into the uses and limitations of this technique for chemical analysis. Measurement techniques and methods of obtaining derivative spectra are discussed. The degree of polynomial fit on the smoothness of derivative spectra and signal-to-noise ratio is described. Application of UV derivative spectrometry for determination of single and multicomponent analysis is shown. Derivative spectrophotometry possibly improves the selectivity and sensitivity of determination which has been illustrated.

Tuberculosis is a disease caused by bacteria spread from person to person through air. TB usually affects the lungs, but it can also affect other parts of the body, such as brain or kidney. Global surveillance has shown that drug resistant TB is widespread and is now a treat to tuberculosis control programs in many countries. This review describes treatment of tuberculosis and the drug resistance problem in India.

Drug resistance in tuberculosis has been shown to result from spontaneous mutation in several chromosomal genes of M.Tuberculosis. Mutation may reduce the medications' capacity to bind to the target genes. In many patients polydrug resistance, multidrug resistance, rifampicin resistance (RR) and extensive drug resistance (XDR) were seen. The diagnosis of drug-resistant TB in HIV-positive persons is more difficult and may be confused with other pulmonary or systemic infections. Management of patients with mono- or poly-resistant TB will be done with standard first line chemotherapy. Treatment of latent infection for people suffering from multidrug resistant bacilli is problematic because the only cure by isoniazid and rifampicin. In the recent cases of severe hepatotoxicity associated with preventive treatment comprising either pyrazinamide and rifampicin or pyrazinamide and fluoroquinolone. The use of dilatory fluoroquinolones, such as moxifloxacin, remarkable improved treatment outcomes of XDR-TB.