This study aims to determine the potential of probiotic lactic acid bacteria Lactobacillus fementum NBRC 15885 from tempoyak isolates in Padang Pariaman, West Sumatera (Indonesia) against acidic conditions, bile salts and antimicrobial activity. In this study we analyzed the probiotic resistance to low pH and bile salts, antimicrobial activity against pathogenic bacteria (Staphylococcus aureus, Escherichia coli and Listeria monocytogene). The results showed that Lactobacillus fermentum NBRC 15885 from tempoyak isolates in Padang Pariaman Regency, West Sumatera (Indonesia) has probiotic properties because it has the ability to resist acidic condition with pH 3.0 with viability of 34%, against bile salts 0.3% with a viability of 97%, and has antimicrobial properties which able to inhibit pathogenic bacteria with a 16 mm inhibition zone in Escherichia coli, 14 mm in Staphylococcus aureus, 10 mm in Listeria monocytogene.
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