Objective: To analyze antibacterial activity of essential oil extracted from Curcuma xanthorrhiza Roxb. rhizomes against bacteria causing pulp necrosis, Enterococcus faecalis, and Fusobacterium nucleatum. Material and Methods: The method used in this research was discdiffusion on MH-A media, based on the standard protocol of National Committee for Clinical Laboratory Standards (NCCLS). This method used 4 replications in each of the study group. The study group consists of 4 different concentration groups (25%, 50%, 75% and 100%) of Temulawak rhizomes essential oil extracts, ChKM and Cresophene were used as the positive control group and DMSO (10%) and Tween 80 (0.5%) was used as the negative control group. Results: The inhibition zones of 25%, 50%, 75%, and 100% of temulawak rhizomes essential oil extracts against E. faecalis were 8.17 mm, 8.54 mm, 9.53 mm and 10.17 mm, respectively. Whereas, the inhibition zones of 25%, 50%, 75% and 100% of temulawak rhizomes essential oil extracts against F. nucleatum were 8.54 mm, 8.95 mm, 9.65 mm and 10.75 mm, respectively. Data analysis using Kruskal-Wallis in both of bacteria showed p=0.00 (p<0.05) and therefore, significant levels of inhibition. Conclusion: Essential oil extracts from C. xanthorrhiza Roxb. rhizomes has medium antibacterial activity against E. faecalis and F. nucleatum.

Adhesion, IL–1β, TNF–α are components that affect in inflammation. So, the effect of steeping green and black Robusta coffee beans to adhesion of <em>Streptococcus mutans</em> on this components. This study used monocytes isolated from healthy human peripheral blood using Ficoll-Hypaque centrifugation method. Monocytes were divided into eight groups, i. e. (i) Control group (untreated monocytes), (ii) <em>Streptococcus mutans</em> group (monocytes + <em>S. mutans</em>), (iii) Black Coffee 2.5 % group (monocytes + black coffee beans 2.5 % + <em>S. mutans</em>), (iv) Black Coffee 5 % group (monocytes + black coffee beans 5 % + <em>S. mutans</em>), (v) black Coffee 10 % group (monocytes + black coffee beans 10 % +  <em>S. mutans</em>), (vi) Green Coffee 2.5 % group (monocytes + green coffee beans 2.5 % + <em>S. mutans</em>), (vii) Green Coffee 5 % group (monocytes + green coffee beans 5 % + <em>S. mutans</em>), (viii) Green coffee 10 % group (monocytes + green coffee beans 10 % + <em>S. mutans</em>). S. mutans adhesion on monocytes was analyzed using histochemistry method, while immunocytochemical staining was used for analyzing IL–1β and TNF–α. Cells counting was done per 100 monocytes under a light microscope with 400 × magnification. Data were analyzed using ANOVA followed by LSD test. Results showed that steeping green and black Robusta coffee beans increased the adhesion of S. mutans on monocytes, but it decreased of IL–1β, TNF–α expression (<em>P</em> &lt; 0.05). In conclusion, steeping of green and black robusta coffee beans reduced inflammation against <em>S. mutans</em>.

Coffee contains active compounds that alleged to have antifungal activity. This study aimed to analyze inhibitory effect of steeping freeze-dried Robusta ground coffee (SFDRGC) to the growth of oral microorganism Candida albicans (the main causative agent of oro-pharyngeal opportunistic infection). This is an in vitro experimental study using the post-test only control group design. Serial dilutions of SFDRGC were studied. An antifungal drug Nystatin was used as a positive control, and sterile distilled water as a negative control. The inhibitory effect was study by means of Agar well diffusi on method. Result showed that SFDRGC demonstrated antifungal activity. The higher concentration of SFDRGC showed the higher antifungal activities significantly (P < 0.05). Concentration of 100% SFDRGC showed anti-candida activity as potent as Nystatin. In conclusion, 100% SFDRGC demonstrated a potent antifungal agent against C. albicans. This study suggested that coffee beverage might be used as anti-candida mouthwash to prevent the risk of oral opportunistic infection. Further studies, however are needed to confirm this notion, such as epidemiological, and phytochemical studies of antifungal bioactive components in coffee that are processed using various methods.

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Ulcer is a lesion that is often found in the oral cavity that causes discomfort, pain, difficulty speaking and difficulty eating. Nowdays, herbal ingredients are widely used as alternative medicine choice besides drugs on the market. Pandan leaf contains flavonoids, alkaloids, saponins, and polyphenols which have antiinflammatory, antibacterial, and antioxidant abilities, allowing faster healing of ulcers. Purpose of this study was to determine the effect of pandanus leaf extract gel on healing ulcers and determine the most effective concentration in healing ulcers. This study used 25 Wistar rats divided into 5 groups, which were applied CMC-Na gel, aloclair gel, 25%, 50% dan 75% pandan wangi leaf extract gel. Each rat was made ulcer by heating the stopper amalgam on the bunsen fire for 30 seconds, then touching to the left buccal mucosa for 1 second, and waiting for 2x24 hours to form an ulcer. Giving the gel every morning and evening and measure the diameter of ulcers every day. This study showed healing time and decrease ulcer diameter pandanus leaf extract gel faster than negative control. The average recovery day was negative control for 9.8 days, positive control for 8 days, pandanus leaf extract gel concentration 25% for 8.6 days, concentration 50% for 8 days, and concentration 75% for 6.8 days. Pandan leaf extract gel accelerated ulcer healing and 75% concentration is the most effective.