Background: Age estimation is of prime importance in forensic science and clinical dentistry. In children, age estimation can be done by skeletal maturity indicators like hand-wrist radiographs and dental age estimation. Skeletal maturity indicators compared with dental age estimation is limited as they are influenced by various environmental parameters, whereas calcification of teeth depends on genes rather than environmental factors. Many of the dental age estimation methods use extracted teeth, which are quite invasive, whereas Cameriere's method is a recently introduced radiographic method first in European population; where in age estimation is done using open apices of teeth by orthopantomogram (OPG). Indian-specific formula was introduced later using permanent mandibular teeth on left side of jaw. The present study aimed to estimate the age and also to determine the accuracy of Cameriere's method using Indian-specific formula on both right and left sides of mandible in Khammam population of South India and also to determine the side which can be efficient in determining age. Methodology: The present study comprised radiographs of 50 subjects (25 boys and 25 girls) ranging from 5 to 15 years. The soft copies of the radiographs of selected subjects were retrieved from the computer attached to the digital orthopantomogram machine (Orthophos XG5; Sirona Dental Systems). The 7 left and right permanent mandibular teeth were assessed in OPGs. The number of teeth with closed apical and with open apical ends of roots was examined and measured. The values were tabulated based on the Cameriere method of age estimation using Indian-specific formula. Statistical analysis was done using paired t test and Karl Pearson's correlation coefficient test. Results: Comparison of dental age with chronological age in males showed non-significant results on both left and right sides of the OPG with a p value of 0.3765 and 0.3045, respectively. Likewise in females, p values of 0.2167 and 0.8089 were noted. When males and females were compared, non-significant results were obtained with a p value of 0.1613 in the age estimated on the left side of the OPG and a p value of 0.4322 on the right side of the OPG. Correlation test showed that left side of the OPG showed better results in determining age than the right side of the OPG with an r value of 0.9982 and 0.9485 in males and females, respectively.

Background: Stathmin is an intracellular phosphoprotein that controls the microtubule dynamics by further regulating proper attachment and alignment of chromosomes in a dividing cell. Thus, any mutation or aberrantly expressed protein that reduces the fidelity of spindle assembly will enhance chromosomal instability contributing to aneuploidy. Oral Squamous Cell Carcinoma is an extensively studied malignancy that occurs due to accumulated genetic changes due to carcinogens. The current study is done to evaluate the stathmin role and its expression in OSCC and Oral epithelial dysplasia (OED). Objective: The aim of the present study is to evaluate the role of stathmin in OSCC and Oral dysplasia and also to correlate the expression of Stathmin with respect to the different histopathological grades of OED and OSCC. Materials and Methods: 30 neutral buffered formalin fixed, paraffin embedded (FFPE) tissues of Oral Leukoplakia/ OED and 30 FFPE tissues of OSCC were subjected to immunohistochemistry with stathmin antibody. Five fields of each case with 300 cells were examined and a mean percentage of positive-stained slides were determined. The percentages were recorded accordingly with their respective histological grades. The results were analysed statistically. Results: The results of the present study demonstrated higher mean values of stathmin in tissues with OSCC (2.50) compared to leukoplakia (2.11) and normal tissues (0.00) with a high level of statistical significance (0.0001). There is also an increase in the percentage levels of stathmin with increase in the histological grade of differentiation in OSCC as well as leukoplakia. Conclusion: The present study found a statistical correlation between increased grades of the disease with expression levels of stathmin. This confirms that stathmin expression can contribute to disease progression and that stathmin might have a potential role as an early diagnostic biomarker and can be a therapeutic target for OSCC.

Background and Objectives: Stathmin is a phosphoprotein, which in its phosphorylated/unphosphorylated states plays a major role in polymerization/depolymerization of microtubules, respectively. Assembly of microtubules is an important aspect of cell division called mitosis. Hinderance in the function of stathmin could lead to damage in the mitotic process resulting in aneuploidy which is common manifestation of malignancies. Hence, stathmin could be used as a tumor marker for oral dysplasias and cancers. The purpose of the study is to compare the expression of stathmin in normal subjects to the patients with oral leukoplakia and to correlate its expression with different histopathological grades of oral leukoplakia This is the first ever study conducted to examine the expression of stathmin in oral dysplasia. Methodology: Thirty histopathologically confirmed cases of oral dysplasia were selected for the study. These tissues were evaluated immunohistochemically for stathmin. To enumerate the stathmin stained cells, 300 cells were examined manually in at least 5 areas and a mean percentage of positive–stained slides were determined. Then, each sample was assigned to one of the following staining scores: (0) – (<10% of stained cells); (1) – (11%–25% of stained cells); (2) – (26%–50% of stained cells); (3) – (51%–75% of stained cells); (4) – (76%–90% of stained cells) and (5) – (91%–100% of stained cells). The results were analyzed statistically using ANNOVA test. Results: When comparison was made with respect to staining scores of stathmin between normal and dysplasia groups, the results were found to be statistically significant with a P = 0.0001. A statistically significant difference was observed between various histopathological grades of dysplasia with respect to stathmin immunohistochemistry scores with a P = 0.0001. Conclusion: These results suggest stathmin as a tumor marker and prognostic indicator.

Introduction: Histological stains are dyes that bind to a variety of tissues. Modified Gallego's (MG) stain is a modification of Lille's stain that can be used as a differential stain for identification of hard tissues in oral pathological lesions. Objectives: The objective of this study was to identify the presence of hard tissues such as enamel, dentin and cementum in normal extracted teeth and odontogenic tumors using MG stain and to compare the efficacy of MG stain with hematoxylin and eosin (H&E) stain. Methods: A total of fifty samples, twenty decalcified sections of teeth and thirty cases of odontogenic tumors, were included in the present study. Two sections were cut from the above cases and stained with H&E stain and MG stain, respectively, and assessed for the nature of hard tissue. Results: In H&E staining, enamel, dentine, cementum and bone stained pink. Whereas, in MG stain, enamel stained pink, dentin and bone stained green, while cementum stained red. The shade of color differs with the degree of mineralization of the hard tissues in MG stain. Conclusion: MG stain can be used as a differential stain for different hard-tissue structures when compared to routine H and E staining.