Asteraceae is the largest and most diverse family of flowering plants which contains more than 20,000 species distributed in nearly all types of habitats all over the world. In mountainous regions such as Mount Bisma, it is estimated to have plenty and diverse member of the Asteraceae family, and used in the local community for various uses. This research aimed to understand the diversity, abundance, and uses of Asteraceae members that are found wild in Mount Bisma. Taxonomy and ecological data were gathered using an exploration method and purposive sampling method, from the point, a plot measured 3x3 m2 was created to estimate the vegetation parameters in the mountain top and mountain valley area. Ethnobotanical data were gathered in Sikunang Village, a nearby village of Mount Bisma using a semi-structured interview and open-ended questions. Data were analysed descriptively and quantitatively using several indices such as Importance Value Index (IVI), Index of Cultural Significance (ICS), and index of Use Value (UV). The result showed that there were 18 species from two subfamilies that grew wild in both mountain top and valley of Mount Bisma. The highest importance value belonged to Ageratina riparia, which was scored in the mountain top and valley 71.00 and 91.53, respectively. Uses of Asteraceae in Sikunang were varies, ranging from being a side dish, medicine, firewood, souvenir, and other uses. Galinsoga parviflora and Galinsoga quadriradiata showed the highest ICS value of 41, whereas Austroeupatorium inulifolium scored the highest in UV of 1.8. The study presented high number of Asteraceae diversity and use. Thus, implies that Mount Bisma has vast unexplored biodiversity and locals around Mount Bisma have rich traditional knowledge.

Phalaenopsis amabilis (L.) Blume commonly called Moth Orchid (Orchidaceae) is a natural orchid species designated as the National Flower of Indonesia for its beautiful flower shape and long-lasting flowering period. Basically, P. amabilis has a long vegetative phase that cause late flowering, about 2 to 3 years for flowering, hence a method to shorten vegetative period is desired. The latest technological approach that can be used to accelerate flowering of P. amabilis is the CRISPR/Cas9 genome editing method to inactivate the GAI (Gibberellic Acid Insensitive) gene as a mutant gene that can accelerate the regulation of FLOWERING TIME (FT) genes flowering biosynthesis pathway. The approach that needs to be taken is to silence the GAI gene with a knockout system which begins with identifying and characterizing the GAI target gene in the P. amabilis which will be used as a single guide RNA. CRISPR/Cas9 mediated knockout efficiency is highly dependent on the properties of the sgRNA used. SgRNA consists of a target sequence, determining its specificity performance. We executed phylogenetic clustering for the PaGAI protein with closely related orchid species such as Dendrobium capra, Dendrobium cultivars and Cymbidium sinensis. SWISS-Model as tool webserver for protein structure homology modeling. Results show that P. amabilis has a specific domain with the occurrence of point mutations in the two conservative domains. Therefore, a single guide RNA reconstruction needs to be implemented.