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Poster session 2, September 22, 2022, 12:30 PM - 1:30 PM Indwelling medical devices are of routine use in hospitals for patient care but they lead to the colonization of microbes on their surface making the patient vulnerable to device-related infections (DRI). Biofilms formed due to the growth of microbes are the most common cause of device-related infections. Objectives To isolate, identify and study the microbial colonization of indwelling medical devices (urinary catheters and central venous catheters). To quantify the biofilm formed by the isolates. Method In the present study, microflora was isolated from urinary catheters (n = 28) and central venous catheters (n = 25) that were catheterized in ICU patients. Quantification of biofilm was done by Crystal violet assay. Results Among 28 urinary catheters, 14 catheters had polymicrobial colonisation (n = 10 mixed bacterial and fungal colonization, n = 3 polybacterial, and n = 1 polyfungal), 10 catheters were observed to have mono-species colonization (n = 6 fungal, n = 4 bacterial), while 4 catheters did not show any colonization. Whereas among 25 central venous catheters, only 3 catheters showed mono-bacterial colonization and 2 catheters showed mono-fungal growth and 20 catheters did not show any colonization. A total of 46 isolates were obtained from all urinary and central venous catheters, out of which Candida spp. was found to be highest in number with n = 13 and 30% of total isolates obtained (the Fig. 1 shows the distribution of microflora obtained). The interesting observation in the study is that Candida spp. is found to have multi-species colonization with bacterial isolates (Pseudomonas aeuroginosa, Klebsiella pneumoniae, Staphylococcus haemolyticus, Enterococcus faecium, and Stenotrophomonas maltophilia) as well as fungal isolates (Trichosporon asahii). Quantification of biofilm formed by these isolates was carried out by crystal violet assay and it was observed that 35.6% isolates formed strong biofilm of which Candida spp. was a major contributor. It was also observed that Candida spp. was always the stronger biofilm forming partner in mixed species biofilms. Conclusion It was concluded that majority of colonization on indwelling medical devices are polymicrobial and Candida spp. appears as an important partner in polymicrobial colonization. Among all obtained isolates maximum were strong biofilm forming of which Candida spp. were the major contributors.