In this study, nano-micelled curcumin was produced with natural sea salt with a view to comparing the in silico molecular binding affinity of pure curcumin compound to the active site of transthyretin. Using an optical light microscope and an electron microscope, it was found that the structure of the surface and the cross-section of nano-micelled curcumin was significantly different from natural sea salt. In particular, the crystal structure and nano-components in the nano-micelled curcumin were united, and the layer was more strongly stabilized than untreated salts. In the virtual 3D structure, in silico molecular docking study, the ligand binding affinity of nano-micelled curcumin to the transthyretin active site was found to be higher than that of pure curcumin. In addition, a nano-micelled curcumin formula interacted with more amino acid residues of transthyretin domains. The pharmacophore feature of the nano-micelled curcumin also showed more condensed and constrained features than normal curcumin. These results suggest that nano-micelled curcumin may effectively bind to and stabilize transthyretin, thereby regulating transthyretin-related physiological diseases. Collectively, the nano-micelled curcumin process suggests that normal curcumin can be modified more efficiently into the novel bio-functional chemical formula to stabilize the transthyretin structure. Therefore, the nano-micelled curcumin process can be applied to the field of the regulation of Alzheimer's disease.Key words : Binding affinity, curcumin, in silico study, nano-micelling, transthyretin *Corresponding author *Tel : +82-10-4527-3736, Fax : +82-54-420-4461 *E-mail : channok@naver.com This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This study aimed to elucidate compounds from natural plant extracts with the potential to expand women's breast size and boost growth. These compounds should also possess the ability to activate female hormone receptor without any adverse effects on the human body. Since it is traditionally known to be effective in female hormone control and health care, Leonurus japonicus was chosen as a source of compounds with potential female breast enlargement and skin's elasticity-enhancing activity. It was also expected that Leonurine, the main ingredient of the Leonurus japonicus, possessed such bioactivity. The L. japonicus extract was obtained using a polar solvent after hydrothermal extraction, and nano-micelling composition (NXCL-4950) was utilized to ensure penetration of the osmotic extract into the skin and increase blood circulation. Based on the composition of the extract, this study suggests the possibility of developing a new remedy, to be used in the beauty industry, that can improve skin health and elasticity and blood circulation for women, to particularly enhance the shape and texture of breasts.