Microscopic
understanding of interaction between H2O
and MAPbI3 (CH3NH3PbI3) is essential to further improve efficiency and stability of perovskite
solar cells. A complete picture of perovskite from initial physical
uptake of water molecules to final chemical transition to its monohydrate
MAPbI3·H2O is obtained with in situ infrared
spectroscopy, mass monitoring, and X-ray diffraction. Despite strong
affinity of MA to water, MAPbI3 absorbs almost no water
from ambient air. Water molecules penetrate the perovskite lattice
and share the space with MA up to one H2O per MA at high-humidity
levels. However, the interaction between MA and H2O through
hydrogen bonding is not established until the phase transition to
monohydrate where H2O and MA are locked to each other.
This lack of interaction in water-infiltrated perovskite is a result
of dynamic orientational disorder imposed by tetragonal lattice symmetry.
The apparent inertness of H2O along with high stability
of perovskite in an ambient environment provides a solid foundation
for its long-term application in solar cells and optoelectronic devices.
Human milk glycans provide a broad range of carbon sources for gut microbes in infants. Levels of protein glycosylation in human milk vary during lactation and may also be affected by the stages of gestation and lactation and by the secretor status of the mother. This was the first study to evaluate systematically dynamic changes in human milk oligosaccharides and fucosylated N-glycans in the milk of Chinese mothers with different secretor statuses during 6 months of lactation. Given the unique single nucleotide polymorphism site (rs1047781, A385T) on the fucosyltransferase 2 gene among Chinese populations, our report provides a specific insight into the milk glycobiome of Chinese mothers, which may exert effects on the gut microbiota of infants that differ from findings from other study cohorts.
The maternal milk glycobiome is crucial for shaping the gut microbiota of infants. Although high core fucosylation catalyzed by fucosyltransferase 8 (Fut8) is a general feature of human milk glycoproteins, its role in the formation of a healthy microbiota has not been evaluated. In this study, we found that the core-fucosylated N-glycans in milk of Chinese mothers selectively promoted the colonization of specific gut microbial groups, such as Bifidobacterium spp. and Lactobacillus spp. in their breast-fed infants during lactation. Compared with Fut8+/+ (WT) mouse-fed neonates, the offspring fed by Fut8+/− maternal mice had a distinct gut microbial profile, which was featured by a significant reduction of Lactobacillus spp., Bacteroides spp., and Bifidobacterium spp. and increased abundance of members of the Lachnospiraceae NK4A136 group and Akkermansia spp. Moreover, these offspring mice showed a lower proportion of splenic CD19+ CD69+ B lymphocytes and attenuated humoral immune responses upon ovalbumin (OVA) immunization. In vitro studies demonstrated that the chemically synthesized core-fucosylated oligosaccharides possessed the ability to promote the growth of tested Bifidobacterium and Lactobacillus strains in minimal medium. The resulting L-fucose metabolites, lactate and 1,2-propanediol, could promote the activation of B cells via the B cell receptor (BCR)-mediated signaling pathway.
IMPORTANCE This study provides novel evidence for the critical role of maternal milk protein glycosylation in shaping early-life gut microbiota and promoting B cell activation of neonates. The special core-fucosylated oligosaccharides might be promising prebiotics for the personalized nutrition of infants.
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