In order to greatly improve the physical function of martial arts athletes, this topic studies the effect of high-protein food on the physical function of martial arts athletes. Forty-five athletes in martial arts events took 5 g of high-protein food every day, 6 times a week for 4weeks, and the left and right forearm, calcaneus bone mineral density, and venous blood was drawn to detect bone metabolism and biochemical indicators related to physical function. The experimental results showed that the bone mineral density of the right calcaneus of male martial arts athletes increased significantly after taking high-protein food, and the bone mineral density of left and right forearms and calcaneus of female martial arts athletes increased significantly. After taking high-protein food, the serum calcium and phosphorus of female athletes and the serum calcium of male athletes were significantly increased. Sex decreased, female athletes significantly decreased serum creatine kinase, and male athletes significantly increased IgM. It can be seen that taking high-protein food for 4 weeks has a certain improvement effect on the bone mineral density of female athletes’ forearm and calcaneus, but has little effect on the bone mineral density of male athletes’ forearm and calcaneus. It can be concluded that high-protein food has no adverse effect on athletes’ bone metabolism, blood biochemical indexes, and immune globulin, and can better maintain the physical function level of martial arts athletes.