BackgroundThe aberrant expression of microRNA-139-3p (miR-139-3p) has been recently involved in the development of multiple tumor types, but its function in ovarian cancer remains not well investigated. In this study, we mainly investigated the function of miR-139-3p in the progression of ovarian cancer.MethodsThe levels of miR-139-3p in ovarian cancer cells and tissues were detected using quantitative real-time-PCR (qRT-PCR) assay. The proliferation, colony formation, migration and invasion of ovarian cancer cell were determined, respectively. A luciferase reporter assay was used to confirm ELAV Like RNA Binding Protein 1 (ELAVL1) was a target gene of miR-139-3p. The expression of ELAVL1 was detected using Western blotting and immunofluorescence staining assay. The roles of miR-139-3p on the growth and metastasis of ovarian cancer cell in vivo were explored using transplanted tumor model and experimental lung metastasis model.ResultsMiR-139-3p was down-regulated in ovarian cancer tissues and ovarian cancer cell lines (SK-OV-3, A2780 and OVCAR-3). Overexpression of miR-139-3p decreased the growth, colony formation, migration and invasiveness of SK-OV-3 and OVCAR-3 cells. Moreover, overexpression of miR-139-3p reduced the growth and lung metastasis of ovarian cancer cells in vivo. The luciferase reporter gene assay indicated that ELAVL1 was a target of miR-139-3p and its expression was negatively regulated by miR-139-3p. Furthermore, the expression of ELAVL1 was inversely correlated with miR-139-3p level in ovarian cancer tissue.ConclusionTaken together, we demonstrated that miR-139-3p regulated ovarian cancer growth and metastasis by modulating the expression of ELAVL1.

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We select a large coal waste rock dumps in Guangxi Heshan , study the characteristics of the distribution of polycyclic aromatic hydrocarbons (PAHs) in its surrounding soil and carry on a soil quality evaluation. Soil samples were collected in different distance and different depth from the dumps. After drying and filtrating the samples, we use the gas chromatography - mass spectrometry (GC - MS) technology for quantitative analysis of 16 kinds of priority pollutions of PAHs. The results show that in the plane, from the near to the distant, the content of PAHs in soil is from much to little. In vertical, from shallow to deep, the content of PAHs degressive changes. And the trend of change is from fast to slow in vertical and level. The plane distribution of PAHs in soil is mainly controlled by wind direction and slope, vertical distribution is controlled by its solubility in water and soil properties.Many kinds of PAHs in soils out of limit, which is poor soil.The 6 kinds of heavy ring PAHs which is carcinogenic is distributed in 5~15 cm of the topsoil.