The reliability and stability of array slides are a big concern for array vendors and end users. Herein, we report on a new type of array slide with high reactivity toward DNA probes and low side reaction. A one-step surface reaction via the Michael addition involved in preparing array slides was developed and characterized by x-ray photoelectron spectroscopy, contact angle, and fluorescence labeling. The effects of array fabrication and storage conditions, i.e., spotting solution pH, high humidity, and long-term storage on the reactivity of the slides were examined. The fabricated DNA arrays could realize good hybridization efficiency (38.2% for slides with 0.88 pmol/cm2), low limit of detection (4 × 10−14M), as well as high mismatch selectivity.

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An efficient route is proposed to synthesize magnetic nitrogen-doped mesoporous carbon (Fe3O4/NMC) composites. The nitrogen-doped mesoporous carbon (NMC) material was prepared using a melamine–formaldehyde resin as nitrogen and carbon source and fumed silica as a template via a template method, and Fe3O4 nanoparticles were introduced via an in situ formation method. The effects of the loading amount of Fe3O4 on the morphology, structure, magnetic properties and adsorption performance of the Fe3O4/NMC composites were investigated. Fe3O4 nanoparticles dispersed well on the surface and inside the NMC, and the Fe3O4/NMC remained a mesoporous structure. Fe3O4/NMC exhibited superparamagnetic behavior, and the maximum saturation magnetization was 30.1[Formula: see text]emu/g. Fe3O4/NMC had a decreased specific surface area and pore volume compared to NMC, but the Fe3O4/NMC composites still showed excellent adsorption capacity for Ag(I), reaching 71.9[Formula: see text]mg/g. The adsorption efficiency of Fe3O4/NMC remained at 90.3% of the initial removal capability after five cycles, which demonstrated the potential large-scale industrial applications of these composites.

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