Although soil microbes play an important role in the functioning of the forest ecosystem, our understanding of the spatial distribution characteristics of soil microbes among different vegetation types in urban forest ecosystems is poor. In this study, with the help of high-throughput sequencing, we examined the vegetation type preferences of soil microbes (fungi and bacteria) and then analyzed the microbe–environment (plant community, light availability, soil properties) relations in a temperate urban forest in China. Our results showed that the soil microbial (bacterial and fungal) richness of deciduous forest was higher than that of evergreen, and mixed forests. The spatial distribution of fungi was more specialized than that of bacteria among different vegetation types. The driving forces of environmental factors on soil bacteria and fungi were different. Our findings suggest that different vegetation types favor the occurrence of different microbes, and the relationships between soil microbes and environmental factors depend on different vegetation types in this temperate urban forest. These findings shed new light on the biodiversity conservation of microbes in temperate urban forests and point to the potential importance of vegetation types for microbe formation.

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The mechanisms responsible for biodiversity formation and maintenance are central themes in biodiversity conservation. However, the relationships between community assembly, phylogeny, and functional traits remain poorly understood, especially following disturbance. In this study, we examined forest community assembly mechanisms in different disturbance regimes across spatial scales and including tree life history classes, using phylogenetic and functional trait metrics. Across disturbance regimes, phylogenetic structure tended to be over-dispersed, while functional structure tended to be clustered. The over-dispersion of phylogenetic structure also increased from small to large diameter species. Moreover, the explanation of spatial distance for the turnover of phylogenetic and functional structure was increased, while environmental distance explained less structure as disturbance intensity decreased. Our findings suggest that niche theory largely explains forest community assembly in different disturbance regimes. Furthermore, environmental filtering plays a major role in moderate to high disturbance regimes, while competitive exclusion is more important in undisturbed and slightly disturbed ecosystems.

The hydrolysis acidification tank mainly relies on microorganisms to treat oily sewage, but in many cases the COD of the effluent from the hydrolysis acidification tank does not decrease or even increase. In this paper, about 50 L of oily wastewater is treated in a facultative anaerobic hydrolysis acidification tank with a temperature of 29 °C, pH 6, high-throughput sequencing technology analyzes found that after long-term operation of the hydrolysis and acidification tank, the dominant bacterial Pseudomonas accounted for only 2.87%, at this time, the effluent COD of the hydrolysis and acidification tank was 450 mg/L. Pseudomonas stutzeri LH-42 a strain screened in the laboratory, which was domesticated and colonized in the hydrolysis acidification tank. High-throughput sequencing and bioinformatics analysis showed that the proportion of Pseudomonas in the hydrolysis acidification tank reached 5.89%, the effluent COD of the hydrolysis and acidification tank was 200 mg/L. The above results indicate the importance of the proportion of Pseudomonas in the hydrolysis and acidification tank to the COD degradation of oily wastewater.

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