Lighting condition is essential to human performance. With the widespread use of computer-based learning, the performance measurements become difficult, and the effects of artificial lighting conditions towards the new learning forms are not investigated extensively. The current study conducts a subject-within experiment with a 45-min-long online learning along with electroencephalogram (EEG)-based measurements, and a post-interview under five lighting setups respectively (300 lx, 3000 K; 300 lx, 4000 K; 300 lx, 6500 K; 500 lx, 4000 K; 1000 lx, 4000 K). Attention is chosen as the key factor to represent the learning performance. The results show that the attention of people aged in the 20s is not affected by the experimental lighting conditions. The results also demonstrate that people in high illumination at 1000 lx are more inclined to sustain attention despite the discomfort and dissatisfaction. Taking the EEG-based attention measurements and post-interview answers into consideration, lighting conditions at 300 lx, 4000 K are the recommended set points for university architectures among the investigated conditions, providing a practical basis when adjusting the lighting standard for its advantage in energy saving. under 500 lx is acceptable without compromising the user experience [11]. Young Adults are more likely to preserve a negative mood under low color temperatures while accomplishing cognitive tasks [12]. On the contrary, high correlated color temperature fluorescent lights seem to improve wellbeing and productivity based on Mills's work [13]. When the light is blue-enriched, which means a relatively high color temperature, subjective alertness, performance, and evening fatigue would be significantly improved [14]. Smolders et al. draws an opposite conclusion that no significant activating effects and even subtle performance-undermining effects in the relatively high CCT condition [15]. These lighting settings formed a wild feature space, while some set points are not common in practical scenes. When studying the learning performance, the realistic lighting setting scale of university buildings should be supported by further study.It can be seen that different experiment settings lead to different conclusions on subjective performance and mental status. However, no matter whether the experiment is field-based or artificial chamber-based, the performance assessments are all based on test scores. These assessments are not suitable for the work, which requires comprehension and imagination, for example, the learning task because the scores are difficult to be made. The effects of artificial lighting settings on learning performance need to be investigated extensively. In recent research [2,[16][17][18], electroencephalogram (EEG) started to be adopted when examining people's mental state and performance. It is presumed that mapping for the relative Highbeta wave in the temporal lobe can be a biological marker for assessing whether an environment is stressful or not [19]. Besides, thermal pleasure is positi...