Beet necrotic yellow vein virus coat protein (BNYVV CP) gene cloned from BNYVV in Inner Mongolia of China was transferred to sugar beet explants using agrobacterium tumefaciens-mediated method. Transgenic regeneration shoots were induced from petioles and cotyledons tissues of sugar beet. Three sugar beet lines that were fit for genetic transformation experiment were screened, which were Sumfull8-1, Sumfull8-3 and 9718. The experiment results showed that plant regeneration frequency was influenced by many factors, which include hormone compositions and concentration in shoots regeneration medium, culture conditions, sugar beet genotype, original explant types and culture methods. Influence factors of transgenic shoot regeneration frequency for sugar beet explants were discussed in this paper. Efficient genetic transformation system and transgenic shoots regeneration system of sugar beet were established.