In Gram-positive pathogen Staphylococcus aureus , pore-forming toxins (PFTs) such as leukocidins and hemolysins play prominent roles in staphylococcal pathogenesis by killing host immune cells and red blood cells (RBCs). However, it remains unknown which combination of toxin antigens would induce the broadest protective immune response against those toxins. In this study, by targeting six major staphylococcal PFTs (i.e., HlgAB, HlgCB, LukAB, LukED, LukSF-PV, and Hla), we generated ten recombinant toxins or toxin-subunits, three toxoids, and their rabbit antibodies. Using the cytolytic assay for RBCs and polymorphonuclear cells (PMNs), we determined the best combination of toxin antibodies conferring the broadest protection against those staphylococcal PFTs. Although anti-HlgA IgG (HlgA-IgG) showed low cross-reactivity to other toxin components, it was essential to protect rabbit and human RBCs and human PMNs. For the protection of rabbit RBCs, Hla H35L toxoid-IgG was also required, whereas, for human PMNs, LukS-IgG and LukA E323A B-IgG were essential too. When the toxin/toxoid antigens HlgA, LukS-PV, Hla H35L , and LukA E323A B were used to immunize rabbits, they increased rabbit survival; however, they did not block staphylococcal abscess formation in kidneys. Based on these results, we proposed that the combination of HlgA, LukS, Hla H35L , and LukA E323A B is the optimal vaccine component to protect human RBCs and PMNs from staphylococcal PFTs. We also concluded that a successful S. aureus vaccine requires not only those toxin antigens but also other antigens that can induce immune response blocking staphylococcal colonization.

In the past ten years, methane has a greenhouse gas, and its concentration increases by 1% per year, while an estimated worldwide annual landfill cover soil surface from escaping methane is about 20 to 70 Mtpa. Microbial methane oxidation can be carried out about 80% of global consumption of methane, the soil microbial methane oxidation can reduce methane escaping from the soil to the atmosphere. Both in domestic and foreign ash recycling in landfill cover soil behavior has performed for many years, but there is a review of domestic and foreign literature ash, mostly looks at the aspects of physical and chemical properties and heavy metals, there is no assessment of the casing methane oxidation impact. This paper mainly urban incinerator ash as a research object, and after a landfill cover soil mined to study experimentally analyze the impact of ash added methane oxidation right.