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Epigallocatechin‑3‑gallate (EGCG) is a polyphenolic compound extracted and isolated from green tea, which has a variety of important biological activities in vitro and in vivo, including anti‑tumor, anti‑oxidation, anti‑inflammation and lowering blood pressure. The aim of the present study was to investigate the protective effect of EGCG against secondary osteoporosis in a mouse model via the Wnt/β‑catenin signaling pathway. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blotting were used to analyze runt‑related transcription factor 2 and osterix mRNA expression, and the protein expression of cyclin D1, Wnt and β‑catenin, and suppressed peroxisome proliferator‑activated receptor γ protein expression. The protective effect of EGCG against secondary osteoporosis was examined and its potential mechanism was analyzed. Treatment with EGCG significantly decreased serum calcium, urinary calcium, body weight and body fat, and increased leptin levels in mice with secondary osteoporosis. In addition, EGCG treatment significantly inhibited the structure score of articular cartilage and cancellous bone in proximal tibia metaphysis in mice with secondary osteoporosis. Treatment also significantly decreased alkaline phosphatase activity, runt‑related transcription factor 2 and osterix mRNA expression. EGCG also significantly induced the protein expression of cyclin D1, Wnt and β‑catenin, and suppressed peroxisome proliferator‑activated receptor γ protein expression in mice with secondary osteoporosis. Taken together, these results suggest that EGCG may be a possible new drug in clinical settings.

The impact of shear conditions during coagulation on the ultrafiltration permeate flux in a coagulation–ultrafiltration (C–UF) process was investigated.

The formation, breakage, and re-growth of flocs were investigated by using modified flocculation tests and numerical simulation to explore the evolution of floc morphology for different hydraulic retention times. The shorter the aggregation time was, the smaller the flocs produced for the same hydraulic conditions were. Another interesting discovery was that broken flocs that formed in shorter aggregation time had the capacity to completely recover, whereas those formed in a longer amount of time had rather worse reversibility of broken flocs. With the addition of the maximum motion step in the representative two-dimensional diffusion-limited aggregation (DLA) model, there was a transition for flocs from isotropic to anisotropic as the maximum motion step increased. The strength of flocs was mainly affected by the distribution of particles near the aggregated core rather than distant particles. A simplified breakage model, which found that broken flocs provided more chances for diffused particles to access the inner parts of flocs and to be uniformly packed around the aggregated core, was first proposed. Moreover, an important result showed that the floc fragments formed with a larger value of the maximum motion step had more growing sites than did those with a smaller msa value, which was a benefit of following the re-forming procedure.