TiO2/GO nanocomposites were synthesized successfully and exhibited an excellent ability to reduce heavy metal ions in reverse osmosis concentrate.

A putative glutamate decarboxylase (GAD) gene, designated ZmGAD1, was cloned from Zea mays with a combination of reverse-transcriptase polymerase chain reaction (RT-PCR) and bioinformatic approaches. The ZmGAD1 cDNA sequence contained a complete open reading frame encoding a putative protein of 496 amino acids, which contained a pyridoxal-5′-phosphate binding domain and a calmodulin (CaM)-binding domain found in nearly all GADs from plants. Sequence analysis showed that it had highest similarity with rice GAD1. Recombinant ZmGAD1 protein was expressed in Escherichia coli, purified and used to measure enzyme activity, which confirmed ZmGAD1 was really a glutamate decarboxylase gene. Southern blotting analysis suggested that ZmGAD1 was present as a single copy gene in the maize genome. RT-PCR analysis revealed that ZmGAD1 was expressed in all examined tissues including the roots, stems, leaves, ears, and tassels. The expression of the ZmGAD1 gene was upregulated and GAD activity was increased in the leaves and roots after treatment with ABA, MeJA, NaCl, PEG, or cold stress. Several stress-related ciselements were present in the ZmGAD1 promoter cloned from maize genomic DNA. These results suggested that ZmGAD1 might play an important role in responses to abiotic factors and hormone treatments.

Four deep eutectic solvents (DESs) were synthesized, and 5–30% polyethylenimine (PEI) was added to make functional DESs (FDESs) for dynamic absorption experiments of hydrogen sulfide. The synthesized FDESs were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, and nuclear magnetic resonance. The results demonstrated the successful synthesis of FDESs. The interaction between H2S and the FDESs was discussed at a molecular level via the quantum chemical calculations. It was noticed that FDESs prefer chemisorption on H2S. In this work, the 25% PEI/FDES@EG showed the highest desulfurization performance. The effects of H2S concentration and temperature on the desulfurization performance were investigated. It was found that a relatively low temperature (30 °C) was favorable for the absorption of H2S. The 25% PEI/FDES@EG could remove H2S efficiently over a low H2S concentration. Moisture played an important role in the FDES desulfurization system. The absorption/desorption cycle experiment indicated that the FDESs retain their good regeneration performance for at least five times.

Soil salinization is a global problem that limits agricultural productivity and sustainable development. As waste‐derived soil amendments, biochar and organic fertilizer have garnered considerable attention for their ability to improve soil physicochemical properties and contribution to agricultural waste resource recovery. However, comparable data on the effects of biochar and organic fertilizers on the physicochemical properties of saline‐alkali soils are lacking. Therefore, we applied biochar (B1: 5 t ha−1 year−1; B2: 10 t ha−1 year−1; and B3: 20 t ha−1 year−1) and organic fertilizer (OF1: 7.5 t ha−1 year−1 and OF2: 10 t ha−1 year−1) to saline‐alkali soil in the Yellow River Delta (YRD), China, continuously for 3 years. Because of the influence of their application on soil fertility and water‐salt status, maize yield increased by 55.01–62.51% and 15.01–26.67% for the biochar and organic fertilizer treated soils, respectively. Biochar and organic fertilizer increased soil water content, Ca2+, Mg2+, total phosphorus, available phosphorus, total nitrogen,NO3−‐N,NH4+‐N, organic matter, and microbial biomass carbon and nitrogen, while decreasing the sodium adsorption ratio and soil pH. Compared with CK, Na+ and soil salt content were reduced by 3.83–8.16% and 2.45–12.08%, respectively, under biochar treatments and increased by 2.19–5.34% and 12.95–20.02%, respectively, under organic fertilizer treatments. Principal component analysis showed that biochar was more effective than organic fertilizer in increasing SWC and reducing salinity and Na+. Based on the evidence of this study, biochar presents an eco‐friendly agricultural strategy for improving saline‐alkali soils and increasing maize yield in the YRD.

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