The aim of this study was to investigate the associations between the levels of neuron-specific enolase (NSE) and S100B protein and coma duration, and evaluate the optimal cut-off values for prediction coma duration ≥ 72 hours in patients with acute carbon monoxide poisoning (ACOP). A total of 60 patients with ACOP were divided into 3 following groups according to their status of consciousness and coma duration at admission: Awake group [Glasgow Coma Scale score (GCS score) ≥ 13 points], Coma < 72 hours group (GCS score < 13 points and coma duration < 72 h), and Coma ≥ 72 hours group (GCS score < 13 points and coma duration ≥ 72 h). The levels of serum NSE and S100B protein were measured after admission. There were significant differences in GCS score, carbon monoxide (CO) exposure time, NSE, and S100B levels between the Coma ≥ 72 h group and the Awake group, and between the Coma < 72 h group and the Awake group. Significant differences in GCS score, NSE, and S100B levels were also found between Coma ≥ 72 h group and Coma < 72 h group. Correlation analysis showed that NSE and S100B were positively correlated ( r s = 0.590, P < .01); NSE and S100B were negatively correlated with GCS score ( r s = -0.583, r s = -0.590, respectively, both P < .01). The areas under the curve (AUCs) of NSE, S100B, and GCS score to predict the coma duration ≥ 72 hours were 0.754, 0.791, and 0.785, respectively. Pairwise comparisons did not show differences among the 3 groups (all P > .05). The sensitivity and specificity of NSE prediction with a cut-off value of 13 μg/L were 80% and 64%, respectively, and those of S100B prediction with a cut-off value of 0.43 μg/L were 70% and 88%, respectively. The NSE and S100B protein levels were significantly correlated with the degree of impaired consciousness and had the same clinical value in predicting coma duration of ≥ 72 hours in patients with ACOP.

Aim: To investigate the impact of high-dose of perindopril on cardiac function and ACE2/AT2R pathway in rabbits with ischemic cardiac dysfunction. Methods: The thirty rabbits with ischemic cardiac dysfunction were divided into high-dose group (Perindopril, 2 mg/kg/d), low-dose group (Perindopril, 0.66 mg/kg/d), and control groups (Saline, 2 ml/kg/d) by a random number table. After four weeks, we measured the cardiac function, The level of Ang-(1-7) and Ang-(1-9), mRNA expression level of ACE2 and AT2R. Results: The results showed that high dose and low dose of perindopril could improve cardiac function (p < 0.001), and high-dose perindopril had more significant improvement (p = 0.041). After treatment, in high-dose group, mRNA level of ACE2, AT2R in myocardium (p < 0.001) and Ang-(1-9) level in serum (p = 0.012) were higher than low-dose group, while Ang-(1-7) levels in serum didn’t show a significant difference (p = 0.829). LVEF and serum Ang-(1-9) were significantly correlated (p = 0.002), LVEF and ACE2 (p = 0.001), LVEF and AT2R (p = 0.007); however, it was no correlation between LVEF and serum Ang-(1-7) (p = 0.067). Conclusion: A high-dose of perindopril could improve ischemic cardiac dysfunction by ACE2/Ang-(1-9)/Ang-(1-7) pathway.