The imbalance between supply and demand of Artemia cysts in China and around the world is increasing now. Salt lakes in Tibet may contribute to the solution of the problem. In Northern Tibet there are 26 saline lakes whose salinity and temperature may support Artemia survival at an altitude of 4 000-5 100 m. We found Artemia in 15 of these lakes. The saline lakes with Artemia populations mainly belong to the shallow basin lakes, and the majority of these lakes are small in area. The total area of lakes without Artemia is more than 1 000 km 2 . Lake Dangxiong Co (Co means lake in Tibet) was chosen for the intentional introduction of Artemia sinica . In 2004, 850 g of A . sinica cysts, originating from Qinghai, were introduced in the lake. Surveys in 2006-2014 showed that the average abundance of Artemia adults in the lake gradually increased from 20 ind./m 3 in 2006 to 1950 ind./m 3 in 2013. We assume that two subpopulations of A . sinica , separated by depth, may exist in the lake. The new Artemia population caused an increase in the number of species of phytoplankton and heterotrophic protozoa with a decrease of their total abundance. Water transparency also increased. Dominance in phytoplankton passed from cyanobacteria to diatoms. Changes occurred not only in the lake ecosystem; the number of water birds using the lakes also dramatically increased. Preliminary calculations showed that is it possible to harvest at least about 150 t cysts per year from the lake as well as 3.2 thousand tons of frozen or 350 t of dried biomass of adult Artemia .
Acid mine drainage (AMD) is one of the biggest environmental challenges associated with in the mining process. Most of the current research on AMD focuses on developed deposits, whereas there is almost no research on naturally-produced AMD from undeveloped deposits. In this study, river water and AMD were collected to analyze the distribution characteristics of heavy metals and the phytoplankton community. In addition, the environmental risks of heavy metals were evaluated by single-factor pollution index, Nemerow pollution index and health risk assessment model. The results show that the pH of the Rongna River water ranged from 6.52 to 8.46, and the average concentrations of Mn and Ni were 867.37 and 28.44 μg/L, respectively, which exceed the corresponding Grade III Environmental Quality Standard of Surface Water. The results of the environmental health risk assessment show that the river section of the Rongna River was seriously polluted by the heavy metal Mn after AMD confluence, and the health risk assessment indicates that oral ingestion of Mn posed a potential non-carcinogenic risk to children and adults. A total of 35 phytoplankton species were found in the Rongna River. The phytoplankton biomass was negatively correlated with the concentration of major heavy metals, indicating that the heavy metal concentration exceeded the tolerance limit of phytoplankton, thereby affecting their normal growth. Finally, statistical analysis shows that Cu, Zn, Ni, Mn and Cd in the Rongna River were mainly derived from AMD.
The surface sediments of the Rongna River and the surface soils around the Tiegelongnan copper deposit were collected, and the heavy metals Cu, Zn, Pb, Cr, Cd, As, Hg, and Ni were measured for their concentrations and health risk assessment. When the Rongna River passed through the Cu deposit area, the concentrations of Cu, Zn, As, Cd, Ni, and Hg in the surface sediments increased significantly, and the concentrations of Cu, Zn, and As exceeded the corresponding Grade II environmental quality standard. The heavy metals in the soil of the mining area were greater than the background value of the soil in Tibet. The geoaccumulation index indicated that the sediments of the river entering the mining area were very highly polluted by Cu and moderately polluted by Cd and Zn, and the soils in the mining area were moderately polluted by Cu. The potential ecological risk (PER) indices revealed that the sediments of the river entering the mining area had significantly high ecological risks, while the PER of the sediments away from the river section of the mining area was low, and the PER of the soils around the Cu deposit was moderate. The results of the health risk assessment indicated that the noncarcinogenic risks of heavy metals in sediments and soil of the mining area were within the acceptable range for adults and children. However, the carcinogenic risk of As and Cd in the sediment and As in the soil exceeds the relevant national standards, which may pose a certain risk to human health.
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