No abstract

As storage temperature had no influence on the acylated homoserine lactones (AHLs) production, the main AHLs (C8-HSL and C6-HSL) were used as reference standards. We proposed to describe the relationship between AHLs and β-galactosidase of AHL biosensor with an equation. The equation could fit the β-galactosidase data well with a high determination coefficient (0.994) and low root mean squared error (0.016). In all experiment, there was no significant difference (P > 0.05) between the observed and calculated AHL values in chicken meat, when different type of AHLs was used. Hence, based on the equation the AHLs similar to Pseudomonas aeruginosa production can be calculated. This approach simplified the calculation of the AHL production in chicken meat and broth. Based on this method, we found that during bacterial growth, the AHLs accumulate in the exponential phase, but decreased in the stationary phase. The highest AHLs were determined during late exponential and early stationary phase. PRACTICAL APPLICATIONIn present study, we propose to simplify the calculation of the AHL produced by P. aeruginosa in chicken meat and broth based on the microplate bioassay.We developed a nonlinear model to describe the relationship between the β-galactosidase expression of Agrobacterium tumefaeiens KYC55 and AHLs addition. The model can be applied to quantify the AHLs-extraction concentration in the range 0-6 ug/mL.